Halichlorine is a novel L-type Ca(2+) channel inhibitor isolated from the marine sponge Halichondria okadai Kadota

European Journal of Pharmacology
Yoshiki TsubosakaHiroshi Ozaki

Abstract

Halichlorine, isolated from a marine sponge Halichondria okadai Kadota, has a unique structure and its physiological activity is virtually unknown. In the present study, we investigated the direct effect of halichlorine on vascular contractility. In endothelium-denuded rat aorta, while the treatment of halichlorine (0.01-10microM) did not induce vascular contraction, halichlorine (0.01-10microM) dose-dependently inhibited both the steady-state precontractions induced by high K(+) (65.4mM) and phenylephrine (1microM). The vasodilator effect of halichlorine (10microM) on high K(+) (65.4mM)-induced contraction was more potent than that on phenylephrine (1microM)-induced contraction (65.4mM high K(+): 72.7+/-3.4%; 1microM phenylephrine: 34.7+/-2.3%). To investigate the mechanism underlying the suppressive effect of halichlorine on vascular contractility, we examined the effect of halichlorine on intracellular Ca(2+) concentration in vascular smooth muscle with a fluorescent Ca(2+) indicator, fura-2. Treatment of halichlorine (10microM) significantly inhibited the sustained [Ca(2+)](i) elevation induced by high K(+) (65.4mM) (45.3+/-5.5%). Furthermore, current measurements by whole-cell mode patch-clamp recording in rat aortic smoot...Continue Reading

References

Oct 1, 1995·Kidney International·E Perez-Reyes, T Schneider
Nov 8, 1996·The Journal of Biological Chemistry·C A Gurnett, K P Campbell
Jan 1, 1997·Annual Review of Physiology·B NiliusG Droogmans
Dec 22, 1999·Angewandte Chemie·D TraunerS J Danishefsky
Jan 12, 2002·British Journal of Pharmacology·Ian McFadzean, Alan Gibson
Jun 25, 2002·European Journal of Pharmacology·Guei-Jane WangChieh-Fu Chen
May 27, 2003·Cell Calcium·Jianjie Ma, Zui Pan
Aug 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hamish S Christie, Clayton H Heathcock
May 4, 2007·The Journal of Pharmacology and Experimental Therapeutics·Alexandra ZahradníkováIvan Zahradník

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Citations

Jun 22, 2010·Journal of Pharmacological Sciences·Yoshiki TsubosakaHiroshi Ozaki
Dec 24, 2011·Natural Product Reports·John W BluntMichèle R Prinsep
Jan 17, 2014·Natural Product Reports·Ryuichi Sakai, Geoffrey T Swanson
Oct 27, 2011·Organic & Biomolecular Chemistry·Camille GignouxRobert A Stockman
Oct 19, 2013·Chemistry, an Asian Journal·Shu XuHirokazu Arimoto

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