Haloferax volcanii, as a Novel Tool for Producing Mammalian Olfactory Receptors Embedded in Archaeal Lipid Bilayer

Life
Simona LobassoAngela Corcelli

Abstract

The aim of this study was to explore the possibility of using an archaeal microorganism as a host system for expressing mammalian olfactory receptors (ORs). We have selected the archaeon Haloferax volcanii as a cell host system and one of the most extensively investigated OR, namely I7-OR, whose preferred ligands are short-chain aldehydes, such as octanal, heptanal, nonanal. A novel plasmid has been constructed to express the rat I7-OR, fused with a hexahistidine-tag for protein immunodetection. The presence of the recombinant receptor at a membrane level was demonstrated by immunoblot of the membranes isolated from the transgenic archaeal strain. In addition, the lipid composition of archaeonanosomes containing ORs has been characterized in detail by High-Performance Thin-Layer Chromatography (HPTLC) in combination with Matrix-Assisted Laser Desorption Ionization-Time-Of-Flight/Mass Spectrometry (MALDI-TOF/MS) analysis.

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Citations

Dec 22, 2019·Applied Microbiology and Biotechnology·R U HaqueT Allers
Feb 7, 2020·International Journal of Molecular Sciences·Kangsan KimByung-Kwan Cho
Dec 4, 2020·Biotechnology Advances·Kevin PfeiferSimon K-M R Rittmann

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Datasets Mentioned

BETA
M64386

Methods Mentioned

BETA
biosensor
PCR
Protein Assay
electrophoresis
transgenic
biosensors

Software Mentioned

Flex Analysis

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