Halomonas smyrnensis as a cell factory for co-production of PHB and levan

International Journal of Biological Macromolecules
Souha TohmeEbru Toksoy Öner

Abstract

Levan is a fructan type polysaccharide that has long been considered as an industrially important biopolymer however its limited availability is mainly due to the bottlenecks associated with its large-scale production. To overcome such bottlenecks in the commercialization of this very promising polysaccharide, co-production of levan with polyhydroxyalkanoates (PHAs) by halophilic Halomonas smyrnensis cultures has been proposed in this study for the first time. After in silico and in vitro assessment of PHA accumulation, fermentation profiles for levan and PHA concentrations were obtained in the presence of sucrose and glucose and the PHA granules observed by TEM were found to be poly(3-hydroxybutyrate) (PHB) after detailed structural characterization by GC-MS, DSC, FTIR and NMR. Six nutrient limitation strategies based on nitrogen (N) and phosphorus (P) were tested but highest levan and PHB yields were obtained under unlimited conditions. H. smyrnensis is proved to co-produce PHB and levan while using inexpensive carbon sources which is a commercially successful microbial cell factory system showing a great potential in lowering manufacturing costs and aiming for a zero waste policy within the biorefinery concept.

Citations

Feb 11, 2020·International Journal of Systematic and Evolutionary Microbiology·Shehzad Abid KhanMohammad Saeid Hejazi
Jan 7, 2021·Applied Microbiology and Biotechnology·Rui ChengJianfa Zhang
Dec 15, 2020·The Science of the Total Environment·Ayesha Algade AmaduSufeng Wang
Apr 23, 2021·Essays in Biochemistry·Jian-Wen Ye, Guo-Qiang Chen
Aug 23, 2021·International Journal of Biological Macromolecules·Zahra EskandariEbru Toksoy Oner
Dec 3, 2021·Journal of Biomolecular Structure & Dynamics·Habeebat Adekilekun OyewusiFahrul Huyop

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