Halophilic Bacteria of Lunsu Produce an Array of Industrially Important Enzymes with Salt Tolerant Activity

Biochemistry Research International
Sonika GuptaAnuradha Sourirajan

Abstract

The halophilic bacterial isolates SS1, SS2, SS3, SS5, and SS8 were characterized for production of industrially important enzymes like amylase, protease, lipase, and glutaminase. Halophilic bacterial isolates SS1 and SS3 exhibited salt dependent extracellular amylase and protease activities. Both the halophilic isolates SS1 and SS3 exhibited maximum amylase and protease activities in the presence of 1.5 and 1.0 M NaCl, respectively, with the optimum pH 8 and temperature 40°C. SS2 showed maximum extracellular protease and lipase activities in the presence of 0.75 M NaCl, at optimum pH of 7, and temperature 37°C. The glutaminase activity of SS3 increased with increase in concentration of NaCl up to 2.5 M. The optimum pH and temperature for L-glutaminase activity of SS3 was 8 and 40°C, respectively. The combined hydrolytic activities of these halophilic bacterial isolates can be used for bioconversion of organic materials to useful products.

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Citations

Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Seyyed Mojtaba MousaviAli Mohammad Amani
Mar 14, 2019·Microbiology Resource Announcements·Sonika GuptaAnuradha Sourirajan
Oct 8, 2020·Journal, Genetic Engineering & Biotechnology·Sonika GuptaAnuradha Sourirajan
Jun 20, 2020·Applied Microbiology and Biotechnology·Noora Barzkar, Muhammad Sohail
Aug 12, 2021·Anais Da Academia Brasileira De Ciências·Ingrid Georgina OrceVerónica Patricia Irazusta
Aug 28, 2021·International Journal of Molecular Sciences·Nur Sabrina Natasha Abdul RahmanKalaivani Nadarajah

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Methods Mentioned

BETA
amylase
protease assay

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