Halothane attenuates nitric oxide relaxation of rat aortas by competition for the nitric oxide receptor site on soluble guanylyl cyclase

European Journal of Pharmacology
M JingS M Muldoon

Abstract

Endothelial cells play an important role in the regulation of vascular activity through the release of endothelium derived relaxing factor (EDRF) now believed to be nitric oxide (NO). NO and the NO donor drug nitroglycerin relax vascular smooth muscle by stimulating soluble guanylyl cyclase leading to elevation of intracellular levels of cyclic guanosine 3',5'-monophosphate (cGMP). Halothane has been shown to inhibit the action of NO on blood vessels. This study was designed to further investigate the mechanisms by which halothane attenuates NO-induced vascular relaxations. This was done by examining the effects of halothane on nitroglycerin and NO-induced relaxations in the presence and absence of the inhibitors of soluble guanylyl cyclase, methylene blue and 6-anilino-5,8-quinolinedione (LY 83583). Thoracic aortas from anesthetized male Sprague-Dawley rats were excised and cut into rings and the endothelium was removed. The aortic rings were suspended in organ baths containing Krebs solution and equilibrated at their optimal passive tension. When a stable plateau of contraction was produced by EC60 concentrations of norepinephrine, increasing concentrations of nitroglycerin or NO were added to the baths to relax the rings. Th...Continue Reading

References

Jan 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·M A Brandt, K P Conrad
Jan 1, 1988·Anesthesiology·S M MuldoonW Freas
Jan 26, 1993·Biochemical Pharmacology·B MayerK Schmidt
Apr 1, 1994·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·K NakamuraK Mori

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Citations

May 8, 2001·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·M J GriffinV Hannon
Jan 11, 2001·Acta Anaesthesiologica Scandinavica·K Metcalf, B Lisander
Mar 8, 2016·Life Sciences·Wenguo FanHongwen He
Dec 13, 2007·Anesthesiology·Noboru TodaYoshio Hatano

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