PMID: 2107750Mar 1, 1990Paper

Halothane-dependent release of intracellular Ca2+ in blood cells in malignant hyperthermia

The American Journal of Physiology
A KlipM E Elliott

Abstract

The concentration of ionized cytosolic calcium [( Ca2+]i) was determined in peripheral blood mononuclear cells from normal and malignant hyperthermia (MH)-susceptible humans and pigs, using the fluorescent Ca2+ indicator indo-1. [Ca2+]i was slightly but significantly elevated in cells from MH human cells relative to normal cells (198 +/- 18 nM, n = 15, and 146 +/- 14 nM, n = 11, respectively, P less than 0.05). Anesthetic concentrations of halothane in the cell suspension resulted in a rapid increase in [Ca2+]i in cells from both normal and MH humans or pigs. The increases (delta) were more pronounced in cells from MH subjects than from normal individuals (delta at 5.7 mM halothane: 245 +/- 53 vs. 57 +/- 11 nM, respectively) and from MH than from normal pigs (delta of 241 +/- 63 vs. 53 +/- 27 nM, respectively). Removal of extracellular Ca2+ obliterated the delta[Ca2+]i caused by halothane in cells from normal humans or pigs but only decreased by about half the delta[Ca2+]i in cells from MH humans or pigs. In 1,2-bis-(aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-loaded cells, in the absence of extracellular Ca2+, halothane failed to increase [Ca2+]i. This suggests that buffering Cai2+ with BAPTA precludes detection of ...Continue Reading

References

Nov 1, 1979·Muscle & Nerve·E M GallantG A Gronert
May 1, 1977·Clinical and Experimental Pharmacology & Physiology·T E Nelson, M A Denborough
Jan 1, 1988·Muscle & Nerve·J R LópezF A Sreter
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Citations

Sep 1, 1995·In Vitro Cellular & Developmental Biology. Animal·D Ennulat, S A Brown
Jul 1, 1991·Acta Anaesthesiologica Scandinavica·M J SeewaldP A Iaizzo
Mar 30, 2001·American Journal of Veterinary Research·S A BrownS Wilson
Dec 2, 2017·Veterinary Pathology·Wilson YauCathy A Brown
Sep 13, 2001·European Journal of Anaesthesiology·F Wappler
Apr 1, 1993·Free Radical Biology & Medicine·G G Duthie, J R Arthur
Aug 13, 1991·Biochimica Et Biophysica Acta·B A OrserH O'Brodovich
Jun 12, 2021·The Veterinary Quarterly·Ping-Hsien ChouKuan-Sheng Chen

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