Halothane potentiation of hydrogen peroxide-induced inhibition of surfactant synthesis: the role of type II cell energy status

Anesthesia and Analgesia
Alka B PatelB A Holm

Abstract

Small concentrations of inhaled anesthetics can affect Type II cell surfactant production and exacerbate oxidant-mediated lung injury. We hypothesized that inhaled anesthetics augment oxidant-induced Type II pneumocyte dysfunction related to their different effects on cellular adenosine triphosphate (ATP) status. Freshly isolated Type II cells were exposed to different concentrations of hydrogen peroxide (H2O2) in the presence or absence of an in vitro halothane exposure. Cells exposed to 100 microM H2O2 alone demonstrated a 23% decrease in ATP levels and a 32% decrease in phosphatidylcholine (PC) synthesis compared with controls. Halothane alone decreased PC synthesis by only 12% and reduced ATP levels by 20%. However, when exposed to both halothane and H2O2 together, ATP levels decreased by 40%, and PC synthesis rates decreased by 51%. Pretreatment of cells with nicotinamide, an inhibitor of poly adenosine diphosphate ribose polymerase, completely prevented the ATP loss and PC synthesis decline caused by H2O2 alone, but it had no effect on the halothane-augmented portion of the cell injury. These data suggest that the ability of halothane to enhance oxidative damage may be related to its own specific effects on cell energetic...Continue Reading

References

Jul 1, 1990·Toxicology and Applied Pharmacology·M TsuchiyaM Hirakawa
Mar 1, 1989·Journal of Applied Physiology·G M LoewenS Matalon
Dec 1, 1988·Journal of Applied Physiology·B A HolmR H Notter
Feb 1, 1987·Journal of Applied Physiology·S MatalonR H Notter
Nov 1, 1985·Journal of Applied Physiology·B A HolmS Matalon
Oct 1, 1973·British Journal of Anaesthesia·G M HallH Baum
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H Rottenberg
Jun 2, 1983·Biochimica Et Biophysica Acta·J N FinkelsteinD L Shapiro
Nov 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·N J Gross, D M Smith
Mar 1, 1996·The American Journal of Physiology·T Krarup, P B Dunham
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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