Halotolerance mechanisms of the methanotroph Methylomicrobium alcaliphilum.

Biotechnology and Bioengineering
Sergio BordelRaúl Muñoz

Abstract

Methylomicrobium alcaliphilum is an alkaliphilic and halotolerant methanotroph. The physiological responses of M. alcaliphilum to high NaCl concentrations, were studied using RNA sequencing and metabolic modeling. This study revealed that M. alcaliphilum possesses an unusual respiratory chain, in which complex I is replaced by a Na+ extruding NQR complex (highly upregulated under high salinity conditions) and a Na+ driven adenosine triphosphate (ATP) synthase coexists with a conventional H+ driven ATP synthase. A thermodynamic and metabolic model showing the interplay between these different components is presented. Ectoine is the main osmoprotector used by the cells. Ectoine synthesis is activated by the transcription of an ect operon that contains five genes, including the ectoine hydroxylase coding ectD gene. Enzymatic tests revealed that the product of ectD does not have catalytic activity. A new Genome Scale Metabolic Model for M. alcaliphilum revealed a higher flux in the oxidative branch of the pentose phosphate pathway leading to NADPH production and contributing to resistance to oxidative stress.

References

Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A VentosaA Oren
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Jacob LesniakDimitar B Nikolov
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·A V Bogachev, M I Verkhovsky
Oct 11, 2005·Nucleic Acids Research·Ross OverbeekVeronika Vonstein
Jan 4, 2006·Biochemistry·Christian ScholzFranz Xaver Schmid
Jan 27, 2009·Biochimica Et Biophysica Acta·Silke SchmidtVolker Müller
Mar 23, 2010·Metabolic Engineering·Sergio Bordel, Jens Nielsen
Aug 20, 2010·Biophysical Chemistry·Christoph HeldGabriele Sadowski
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Dec 31, 2011·Journal of Bacteriology·Stéphane VuilleumierMarina G Kalyuzhnaya
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Aug 10, 2013·BMC Systems Biology·Ali EbrahimDaniel R Hyduke
Dec 3, 2013·Nucleic Acids Research·Ross OverbeekRick Stevens
Dec 5, 2013·Nature Communications·M G KalyuzhnayaM E Lidstrom
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Apr 15, 2015·The Journal of General Physiology·Janet M Wood
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Raphael Alhadeff, Arieh Warshel
May 6, 2017·Science·Ilissa B OckoStephen W Pacala
Jul 16, 2017·Physical Review. E·Sergio Bordel
Oct 11, 2017·Metabolic Engineering Communications·Austin D ComerBrian F Pfleger
Feb 8, 2018·Scientific Reports·Ilya R AkberdinMarina G Kalyuzhnaya
Oct 20, 2018·Frontiers in Microbiology·Toshihide MatsunoIsao Yumoto
Jul 3, 2019·Microbial Biotechnology·Kyle T WhiddonMichael C Konopka

❮ Previous
Next ❯

Citations

Apr 25, 2021·Applied and Environmental Microbiology·Scott SugdenLisa Y Stein
Aug 9, 2021·Journal of Environmental Management·Alessandro A Carmona-MartínezRaúl Muñoz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.