Hammondia sp. oocysts shed by a Brazilian fox (Lycalopex vetulus) differ from Hammondia heydorni and Hammondia triffittae

Parasitology Research
L F P GondimAnderson L de Carvalho

Abstract

A Brazilian fox (Lycalopex vetulus) was rescued from a highway, and 16 days after maintained in captivity, the fox shed oocysts with sizes compatible with Hammondia sp. and Neospora caninum. DNA extracted from oocysts were initially tested in two PCRs targeting the internal transcribed spacer 1 (ITS-1) of the rDNA of Hammondia heydorni and the Nc-5 gene of N. caninum. A 270-bp product was visualized in the PCR for H. heydorni. No amplification was observed for N. caninum PCR. Since ITS-1-based PCR is not sufficient to differentiate Hammondia species derived from canids, oocyst DNA was examined using multilocus sequence analysis of five genetic fragments [intron 1 of the alpha tubulin gene (intron 1), internal transcribed spaces 1 and 2 (ITS-1 and ITS-2) of the rDNA, 28S rRNA gene (D2/D3 domain), and heat shock protein 70 (Hsp70)]. The Hammondia sp. oocyst from the Brazilian fox, referred here as H-FOXBR isolate, is closely related to H. heydorni and Hammondia triffittae, but differs from these parasites in three genetic markers (alpha tubulin gene, ITS-2, and 28S rRNA). As reported by other research groups, Hammondia spp. excreted by canids are genetically diverse and may encompass additional species besides H. heydorni and H. ...Continue Reading

References

Nov 1, 1977·Infection and Immunity·E Christie, J P Dubey
Aug 1, 1980·Parasitology·J P Dubey, C S Williams
Apr 1, 1996·International Journal for Parasitology·S Payne, J Ellis
May 26, 1999·Parasitology·J T EllisJ P Dubey
Sep 29, 2005·Parasitology·J AbelJ T Ellis
Mar 24, 2009·Veterinary Parasitology·Rodrigo M SoaresHilda F J Pena
Oct 30, 2010·Veterinary Parasitology·Rodrigo Martins SoaresLeonardo José Richtzenhain
Jun 5, 2013·Veterinary Parasitology·Hu Jun JieFu QingTuan

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Datasets Mentioned

BETA
KC515451

Methods Mentioned

BETA
amputation
PCR
PCRs

Software Mentioned

Codoncode aligner
BLAST

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