Handheld impedance biosensor system using engineered proteinaceous receptors

Biomedical Microdevices
Ebrahim Ghafar-ZadehMark Trifiro

Abstract

We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.

References

Oct 24, 1998·Analytical Chemistry·I BontideanN L Brown
Jun 20, 1996·Chemical Reviews·Abraham Ulman
May 15, 2004·Biosensors & Bioelectronics·Dong-Sun KimGeunbae Lim
Aug 2, 2006·Molecular BioSystems·Maria StaianoSabato D'Auria
Apr 19, 2007·Chemical Reviews·Feng Tao, Steven L Bernasek
Aug 5, 2008·Biomedical Microdevices·Abdur Rub Abdur RahmanAnthony Guiseppi-Elie
Dec 3, 2008·Analytical Chemistry·Cheng-Teng HsuJyh-Myng Zen
Dec 17, 2008·Analytical Chemistry·Arghavan ShabaniRosemonde Mandeville
Jan 24, 2009·Biomedical Microdevices·Abdur Rub Abdur Rahman, Anthony Guiseppi-Elie

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Citations

Aug 19, 2014·IEEE Transactions on Biomedical Circuits and Systems·Ghazal NabovatiMohamad Sawan
Jul 10, 2019·Physical Chemistry Chemical Physics : PCCP·F Rafael CastielloMaryam Tabrizian
Jul 6, 2019·Biomedical Microdevices·M Anne Sawhney, R S Conlan

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