Handling of tracer bicarbonate by the liver. The relative impermeability of hepatocyte cell membranes to the ionic species

Circulation Research
A J SchwabC P Rose

Abstract

The multiple indicator dilution technique was used to study transfer of labeled HCO3- across the hepatocyte membrane in the anesthetized mongrel dog. A bolus of H[14C]O3-, 51Cr-labeled erythrocytes, [36Cl-] and/or [3H]sucrose, and [3H]OH was injected through a catheter in the portal vein, and timed anaerobic blood samples were obtained from a catheter in the hepatic vein. Experiments were carried out in untreated controls and after intravenous infusion of acetazolamide (100 mg/kg). In the controls, the H[14C]O3- curve was very similar to the [3H]OH curve. The dilution curves were all linear transformations of each other, indicating that HCO3-, as had previously been shown for the other diffusible tracers, undergoes delayed-wave flow-limited distribution. The distribution space for H[14C]O3- in the control situation includes the blood plasma and interstitial spaces, the erythrocyte interior modified by a Donnan equilibrium, and the available liver cellular space. The calculated HCO3- concentration in the liver cells was somewhat lower than that in the plasma space; the difference implied a cellular pH lower than that of plasma by approximately 0.08 pH units. When the carbonic anhydrases were inhibited with acetazolamide, the dil...Continue Reading

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Citations

Jun 2, 2005·Vascular Pharmacology·Alice M DerbocioEmy L Ishii-Iwamoto
Jul 23, 2013·Clinical Physiology and Functional Imaging·Jens H HenriksenHenrik B W Larsson
May 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C A GoreskyA Bracht

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