PMID: 2123383Jan 1, 1990Paper

Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay

Archives of Virology
Y W TangZ Y Xu

Abstract

Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays.

References

Mar 1, 1978·The Journal of Infectious Diseases·H W LeeK M Johnson
Sep 1, 1986·The Journal of Infectious Diseases·H X ChenS Y Gao
Mar 1, 1987·Virology·C S SchmaljohnJ M Dalrymple
Feb 1, 1983·Japanese Journal of Medical Science & Biology·T KitamuraS Urasawa
Aug 1, 1984·The Journal of General Virology·L H ElliottJ B McCormick
Oct 1, 1982·Journal of Clinical Microbiology·L H ElliottK M Johnson

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Citations

Oct 1, 1992·Virus Research·P PuthavathanaC Y Kang
Oct 31, 2007·Virology Journal·Jin-Won SongRichard Yanagihara
Feb 7, 2008·Emerging Infectious Diseases·Satoru AraiRichard Yanagihara
May 3, 2014·Viruses·Renata Carvalho de OliveiraElba Regina Sampaio de Lemos
Aug 2, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wen-Ping GuoYong-Zhen Zhang

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