Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae

The Journal of Biological Chemistry
Vijayendran RaghevendranJens Nielsen

Abstract

In Saccharomyces cerevisiae, the heme-activated protein complex Hap2/3/4/5 plays a major role in the transcription of genes involved in respiration. Thus, overexpression of HAP4 has been shown to result in a 10% increase in the respiratory capacity. Here the physiology of a HAP4-deleted S. cerevisiae strain was investigated, and we found that the hap4delta S. cerevisiae exhibited poor growth on ethanol, although the growth rate on glucose was indifferent from the wild type in aerobic as well as anaerobic cultures. Moreover, it exhibited a large (75%) reduction in the critical glucose uptake rate at which fermentative metabolism is onset, indicating a substantial reduction in respiratory capacity. We also performed whole genome transcription analysis for the hap4delta and the wild type, grown in carbon-limited chemostat cultures operated at a dilution rate of 0.05 h(-1). Although both strains exhibited respiratory metabolism, there was significant change in expression of many genes in the hap4delta strain. These genes are involved in several different parts of the metabolism, including oxidative stress response, peroxisomal functions, and energy generation. This study strongly indicates that Hap4 activation only occurs at interm...Continue Reading

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Citations

Feb 10, 2009·BMC Systems Biology·Donatella CiminiJens Nielsen
Apr 7, 2010·PLoS Computational Biology·Aleksej ZelezniakKiran Raosaheb Patil
Jul 7, 2012·PloS One·Tugce Bilgin, Andreas Wagner
Oct 7, 2015·Journal of Industrial Microbiology & Biotechnology·Akinori Matsushika, Tamotsu Hoshino
Nov 6, 2009·Molecular Systems Biology·Matthew A OberhardtJason A Papin
Sep 14, 2010·International Journal of Food Microbiology·Rafael Dueñas-SánchezTahía Benítez
Aug 6, 2019·Frontiers in Microbiology·Yinhe Mao, Changbin Chen
Jul 3, 2021·Current Microbiology·Lirong XiangZhijie Tan

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