Haplotype combination of the bovine CFL2 gene sequence variants and association with growth traits in Qinchuan cattle

Gene
Yujia SunHong Chen

Abstract

The aim of this study was to examine the association of cofilin2 (CFL2) gene polymorphisms with growth traits in Chinese Qinchuan cattle. Three single nucleotide polymorphisms (SNPs) were identified in the bovine CFL2 gene using DNA sequencing and (forced) PCR-RFLP methods. These polymorphisms included a missense mutation (NC_007319.5: g. C 2213 G) in exon 4, one synonymous mutation (NC_007319.5: g. T 1694 A) in exon 4, and a mutation (NC_007319.5: g. G 1500 A) in intron 2, respectively. In addition, we evaluated the haplotype frequency and linkage disequilibrium coefficient of three sequence variants in 488 individuals in QC cattle. All the three SNPs in QC cattle belonged to an intermediate level of genetic diversity (0.25<PIC<0.5). Haplotype analysis of three SNPs showed that 8 different haplotypes were identified in all, but only 5 haplotypes were listed except for those with a frequency of <0.03. Hap4 (-GTC-) had the highest haplotype frequencies (34.70%). However in the three SNPs there were no significant associations between the 13 combined genotypes of the CFL2 gene and growth traits. LD analysis showed that the SNP T 1694 A and C 2213 G loci had a strong linkage (r(2)>0.33). Association analysis indicated that SNP G 1...Continue Reading

References

Jan 9, 2001·Trends in Ecology & Evolution·D Posada, K A Crandall
Apr 21, 2001·European Journal of Human Genetics : EJHG·J AkeyM Xiong
Apr 23, 2002·Nature Reviews. Genetics·Kristin G ArdlieMark Seielstad
Oct 18, 2002·Genetic Epidemiology·Richard W Morris, Norman L Kaplan
Nov 27, 2010·American Journal of Human Genetics·Nyamkhishig SambuughinLev G Goldfarb
May 9, 2012·Neuromuscular Disorders : NMD·C W OckeloenN van Alfen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.