PMID: 7082667Jan 26, 1982Paper

Haptoglobin biosynthesis in rats. Immunological identification of polysomes synthesizing haptoglobin and quantitation of haptoglobin in the cytoplasm of liver cells

Biochimica Et Biophysica Acta
F A BagliaG M Fuller


A quantitative enzyme-linked immunosorbent assay was developed and utilized to study the stimulation of haptoglobin biosynthesis during an acute inflammatory challenge. A 10-fold increase in intracellular haptoglobin was measured at the peak of the inflammatory response. The increase in serum haptoglobin levels was concomitant with the intracellular levels, demonstrating the secretory output is also elevated during the inflammatory period. A monospecific antihaptoglobin was produced and used to detect the specific polysomes involved in haptoglobin synthesis. The amount of radioactively labeled antibody bound to the nascent haptoglobin chain was increased approx. 3-fold during the inflammatory response, indicating that new haptoglobin was being synthesized and suggesting an increase in functional haptoglobin mRNA resulting from the inflammatory signal.


Dec 1, 1977·Analytical Biochemistry·S W KwanR M Goldblum
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A KuroskyW M Fitch


Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J C JamiesonB M Woloski
Feb 1, 1997·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·G R UpchurchG Johnson
Jul 22, 2009·Nature Chemical Biology·Edward McDonald
Jun 1, 1986·Inflammation·R PiccolettiA Bernelli-Zazzera
Dec 1, 1986·The American Journal of Physiology·W S LiaoJ M Taylor

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Enzyme-Linked Immunosorbent Assay
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