Haptoglobin expression and release by rabbit oviduct and endometrium, its localization in blastocyst extra-embryonic matrix and fluid during preimplantation time

Human Reproduction
A HerrlerHenning M Beier

Abstract

Evidence is emerging that haptoglobin, an acute phase protein with immunomodulatory properties, is expressed by the endometrium of various species. The present study describes an in-depth investigation of haptoglobin expression and release in the rabbit reproductive tract and in preimplantation embryos. The full-length cDNA sequence of rabbit haptoglobin was determined by rapid amplification of cDNA ends PCR. Haptoglobin expression was studied in the oviductal ampull, and isthmus, endometrium and embryos from the time of ovulation up to adhesion. These results were completed by western blot analysis of reproductive tract secretions and embryonic tissues. cDNA sequencing showed a high homology between rabbit and human haptoglobin (84.1%). In oviductal tissues haptoglobin mRNA is clearly expressed from 6 h post-conception (p.c.) to day 3, and in the uterus on days 5 and 6. In the oviductal fluid highest haptoglobin protein content was found between 6 h p.c and day 2, and in the uterine fluid on days 5 and 6 p.c. Embryos do not express haptoglobin mRNA during preimplantation development. However, considerable amounts of maternal haptoglobin protein were detected in the blastocyst coverings and in blastocyst fluid. Already during p...Continue Reading

References

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Dec 13, 2002·Anatomy and Embryology·A HerrlerH M Beier
May 30, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·K LaveryG Killian

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Citations

Dec 19, 2013·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Tracey L WeissgerberUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
Apr 10, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·F Gloria-BottiniE Bottini
Aug 18, 2005·Molecular & Cellular Proteomics : MCP·A Stephen GeorgiouAlireza Fazeli
Jul 11, 2008·Biology of Reproduction·Ellen Menkhorst, Lynne Selwood
Aug 29, 2020·Biology of Reproduction·Xi GuoXiaoyan Chen
Nov 17, 2007·Journal of Proteome Research·A Stephen GeorgiouAlireza Fazeli
Jun 10, 2021·Scientific Reports·Francisco A García-VázquezMª José Izquierdo-Rico

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