PMID: 9523852Apr 2, 1998Paper

Haptotactic and growth stimulatory effects of fibrin(ogen) and thrombin on cultured fibroblasts

The Journal of Laboratory and Clinical Medicine
Raphael GorodetskyG Marx

Abstract

We tested the ability of purified, ultraviolet C virally inactivated components of human fibrin sealant (FS) to modulate the chemotaxis, adherence, and proliferation of cultured cells. A fibrin clot formed on a near-confluent layer of human fibroblasts (HFs) recruited cells from the surrounding area. Thrombin (Thr) enhanced HF proliferation by a factor of 1.5 to 1.8, whereas fibrinogen (Fib) exerted only a minimal proliferative effect. We developed a new cell haptotactic/attachment assay by using Thr and Fib covalently bound to Sepharose beads (SBs). The kinetics of cell binding were approximately equivalent for beads coated with either protein. Uncoated SBs or fibrinogen-bound SBs (Fib-SB) pretreated with plasmin did not attract HFs. AlphaThr-SB induced a positive migratory response that was not affected by blocking its proteolytic site, whereas gammaThr-SB elicited no response. X irradiation of HFs at a dose of 6 Gy showed that the migratory response of HF is independent of proliferation, as confirmed by a bromodeoxyuridine uptake assay. Several types of cultured cells (murine fibroblasts, smooth muscle cells, aortic endothelial cells, and murine mammary carcinoma cells) also attached to Fib-SB. By contrast, human keratinocyt...Continue Reading

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Citations

Mar 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raphael GorodetskyGerard Marx
Jun 12, 2003·Experimental Cell Research·Raphael GorodetskyGerard Marx
Jul 14, 2001·Colloids and Surfaces. B, Biointerfaces·H NygrenN Yahyapour
Feb 20, 2013·ACS Chemical Neuroscience·Gerard Marx, Chaim Gilon
Sep 14, 2010·The Journal of Allergy and Clinical Immunology·Maamoun BasheerRaphael Gorodetsky
Sep 14, 2010·Biochemical and Biophysical Research Communications·Liron Levy-BeladevRaphael Gorodetsky
Sep 7, 2012·The Journal of Investigative Dermatology·Michael W XieWilliam H McBride
Feb 21, 2006·The Urologic Clinics of North America·L Andrew Evans, Allen F Morey
Feb 24, 2012·Journal of Biomedical Materials Research. Part a·Alfred GugerellWaltraud Pasteiner
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raphael GorodetskyGerard Marx
Jul 20, 2002·The Journal of Urology·Allen F MoreyJohn P Foley
May 7, 2010·Clinical Oral Implants Research·Volker GasslingStephan T Becker
Nov 8, 2008·Expert Opinion on Biological Therapy·Raphael Gorodetsky
Dec 24, 2011·International Journal of Biomaterials·A StolzingA Scutt
May 3, 2006·International Braz J Urol : Official Journal of the Brazilian Society of Urology·L Andrew Evans, Allen F Morey
Jun 27, 2003·Journal of Surgical Oncology·Dale DantzerRobert S Bell
Mar 27, 2021·Urologia·Sunirmal ChoudhuryDilip Kumar Pal
Jun 26, 1999·The Journal of Investigative Dermatology·R GorodetskyG Marx
Sep 25, 2002·The Journal of Laboratory and Clinical Medicine·Gerard Marx, Xiaode Mou

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