Hard alpha-keratin degradation inside a tissue under high flux X-ray synchrotron micro-beam: a multi-scale time-resolved study

Journal of Structural Biology
Emilie LecciaFatma Briki

Abstract

X-rays interact strongly with biological organisms. Synchrotron radiation sources deliver very intense X-ray photon fluxes within micro- or submicro cross-section beams, resulting in doses larger than the MGy. The relevance of synchrotron radiation analyses of biological materials is therefore questionable since such doses, million times higher than the ones used in radiotherapy, can cause huge damages in tissues, with regard to not only DNA, but also proteic and lipid organizations. Very few data concerning the effect of very high X-ray doses in tissues are available in the literature. We present here an analysis of the structural phenomena which occur when the model tissue of human hair is irradiated by a synchrotron X-ray micro-beam. The choice of hair is supported by its hierarchical and partially ordered keratin structure which can be analysed inside the tissue by X-ray diffraction. To assess the damages caused by hard X-ray micro-beams (1 microm(2) cross-section), short exposure time scattering SAXS/WAXS patterns have been recorded at beamline ID13 (ESRF) after various irradiation times. Various modifications of the scattering patterns are observed, they provide fine insight of the radiation damages at various hierarchica...Continue Reading

References

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Citations

Feb 26, 2016·ACS Nano·Clément Y J HémonnotSarah Köster
Sep 3, 2020·Journal of Synchrotron Radiation·Artur D SurowkaL Vaccari
Feb 11, 2021·Journal of Synchrotron Radiation·Chiara CassiniSarah Köster
Aug 9, 2017·ACS Nano·Clément Y J Hémonnot, Sarah Köster

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