Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline.

Platelets
I C A MunnixY M C Henskens

Abstract

Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national reference values for LTA. The SSC guideline was used as base for a national procedure. Almost all recommendations of the SSC were followed e.g. no adjustment of PRP, citrate concentration of 109 mM, 21 needle gauge, fasting, resting time for whole blood and PRP, centrifugation time, speed and agonists concentrations. LTA of healthy volunteers was measured in a total of 16 hospitals with 5 hospitals before and after standardization. Results of more than 120 healthy volunteers (maximum aggregation %) were collected, with participating laboratories using 4 different analyzers with different reagents. Use of low agonist concentrations showed high variation before and after standardization, with the exception of collagen. For most high agonist concentrations (ADP, collagen, ristocetin, epinephrine and arachidonic acid) variability in healthy subjects decreased after standardi...Continue Reading

References

Mar 1, 2005·Thrombosis and Haemostasis·Karen A MoffatUNKNOWN North American Specialized Coagulation Laboratory Association
Jun 25, 2005·Journal of Clinical Pathology·H ManiE Lindhoff-Last
Jul 14, 2005·Platelets·H K Breddin
Feb 19, 2008·Thrombosis and Haemostasis·Paquita Nurden, Alan T Nurden
Feb 23, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Catherine P M Hayward
Jul 28, 2011·British Journal of Haematology·Paul HarrisonUNKNOWN British Committee for Standards in Haematology
Mar 23, 2013·Journal of Thrombosis and Haemostasis : JTH·S P WatsonUNKNOWN GAPP consortium
Nov 19, 2014·Journal of Thrombosis and Haemostasis : JTH·P Gresele
Sep 30, 2017·Platelets·Corinne FrèreMarie-Christine Alessi
Nov 15, 2017·Platelets·Floor C J I MoenenYvonne M C Henskens
Jan 18, 2018·Journal of Thrombosis and Haemostasis : JTH·I van AstenR T Urbanus
Mar 16, 2018·Platelets·Melissa V ChanTimothy D Warner
May 10, 2018·International Journal of Laboratory Hematology·P GreseleL Bury
Jun 20, 2018·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Joyce P M KrekelsYvonne M C Henskens
Oct 24, 2018·Research and Practice in Thrombosis and Haemostasis·Sean PlattonGary W Moore
Apr 24, 2019·Research and Practice in Thrombosis and Haemostasis·Niklas BoknäsSofia Ramström
Jun 4, 2019·Thrombosis and Haemostasis·Karina AlthausUNKNOWN THROMKID-Plus Studiengruppe der Gesellschaft für Thrombose- und Hämostaseforschung (GTH) und der Gesellschaft für Pädiatrisc

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Citations

Jun 16, 2021·International Journal of Laboratory Hematology·Tinne MonteyneKatrien M J Devreese
Dec 11, 2021·Hematology·Kristi J Smock, Karen A Moser

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