Harnessing an Inhibitory Role of miR-16 in Osteogenesis by Human Mesenchymal Stem Cells for Advanced Scaffold-Based Bone Tissue Engineering

Tissue Engineering. Part a
Irene Mencía CastañoFergal J O'Brien

Abstract

MicroRNA (miRNA) therapeutics is increasingly being developed to either target bone-related diseases such as osteoporosis and osteoarthritis or as the basis for novel bone tissue engineering strategies. A number of miRNAs have been reported as potential osteo-therapeutics but no consensus has yet been established on the optimal target. miR-16 has been studied extensively in nonosteogenic functions and used as functionality reporter target in the development of nonviral miRNA delivery platforms. This study hypothesized that miR-16 may also play an inhibitory role in osteogenesis due to its ability to directly target Smad5 and AcvR2a. This study thus aimed to assess the potential of miR-16 inhibition to increase osteogenesis in human mesenchymal stem cells (hMSCs) using a previously established miRNA delivery platform composed of nanohydroxyapatite (nHA) particles as nonviral vectors in combination with collagen-nHA scaffolds designed specifically for bone repair. Initial results showed that antagomiR-16 delivery efficiently increased the relative levels of both putative targets and Runx2, the key transcription factor for osteogenesis, while also increasing osteocalcin levels. Furthermore, significant increases in mineral calcium...Continue Reading

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Citations

Nov 15, 2018·Tissue Engineering. Part a·Elizabeth R Balmayor, Christopher H Evans
May 31, 2019·Cellular and Molecular Life Sciences : CMLS·Daniele BellaviaG Giavaresi
Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
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Oct 17, 2018·International Journal of Molecular Sciences·Cristina SobacchiCiro Menale
Jun 6, 2019·Biotechnology Journal·Marco A ArriagaSue Anne Chew
Apr 10, 2019·International Journal of Endocrinology·Xi SunXianghang Luo
Jan 1, 2021·Frontiers in Bioengineering and Biotechnology·Yongtao ZhangMostafa El Dafrawy
Jun 26, 2021·Frontiers in Molecular Biosciences·Laila A Damiati, Sarah El-Messeiry

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Methods Mentioned

BETA
transfection
PCR
Assay
genetic modifications
transfect

Software Mentioned

SkanIt ®
Varioskan
NIS Elements

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