Harnessing Redox Cross-Reactivity To Profile Distinct Cysteine Modifications

Journal of the American Chemical Society
Jaimeen D MajmudarBrent R Martin

Abstract

Cysteine S-nitrosation and S-sulfination are naturally occurring post-translational modifications (PTMs) on proteins induced by physiological signals and redox stress. Here we demonstrate that sulfinic acids and nitrosothiols react to form a stable thiosulfonate bond, and leverage this reactivity using sulfinate-linked probes to enrich and annotate hundreds of endogenous S-nitrosated proteins. In physiological buffers, sulfinic acids do not react with iodoacetamide or disulfides, enabling selective alkylation of free thiols and site-specific analysis of S-nitrosation. In parallel, S-nitrosothiol-linked probes enable enrichment and detection of endogenous S-sulfinated proteins, confirming that a single sulfinic acid can react with a nitrosothiol to form a thiosulfonate linkage. Using this approach, we find that hydrogen peroxide addition increases S-sulfination of human DJ-1 (PARK7) at Cys106, whereas Cys46 and Cys53 are fully oxidized to sulfonic acids. Comparative gel-based analysis of different mouse tissues reveals distinct profiles for both S-nitrosation and S-sulfination. Quantitative proteomic analysis demonstrates that both S-nitrosation and S-sulfination are widespread, yet exhibit enhanced occupancy on select proteins,...Continue Reading

References

May 15, 1987·Analytical Biochemistry·C F Babbs, M J Gale
Sep 1, 1995·The American Journal of Physiology·C M Padgett, A R Whorton
Feb 1, 1995·Free Radical Biology & Medicine·R C Scaduto
Aug 12, 1999·Cellular and Molecular Life Sciences : CMLS·M C Broillet
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·S R Jaffrey, S H Snyder
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mayuree Fuangthong, John D Helmann
May 30, 2002·Biological Chemistry·Gregory I Giles, Claus Jacob
Feb 22, 2003·The Journal of Biological Chemistry·David Jourd'heuilMartin Feelisch
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Leslie B PooleAl Claiborne
Jun 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rosa M Canet-AvilésMark R Cookson
Dec 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adrian T SaurinPhilip Eaton
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
Mar 8, 2005·Free Radical Biology & Medicine·Yanhong Zhang, Neil Hogg
Oct 13, 2005·Molecular & Cellular Proteomics : MCP·Jeffrey C SilvaScott J Geromanos
Nov 17, 2005·Bioconjugate Chemistry·Leslie B PooleS Bruce King
Dec 1, 2005·Biochemical and Biophysical Research Communications·Genta ItoTakeshi Iwatsubo
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gisele MonteiroLuis E S Netto
Aug 5, 2008·Nitric Oxide : Biology and Chemistry·Daniela GiustariniRanieri Rossi
Aug 20, 2008·Journal of Neuroscience Research·Eunsung JunnM Maral Mouradian
Sep 23, 2008·Current Opinion in Chemical Biology·Khalilah G Reddie, Kate S Carroll
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
Aug 19, 2009·Free Radical Biology & Medicine·Philipp J KahleThomas Gasser
Mar 17, 2010·Journal of the American Chemical Society·Mahiuddin BaidyaHerbert Mayr
Jul 30, 2010·Inorganic Chemistry·Zachary J TonzetichStephen J Lippard
Nov 20, 2010·Current Opinion in Chemical Biology·Divya Seth, Jonathan S Stamler
Jan 18, 2011·Antioxidants & Redox Signaling·Jenna F DuMond, S Bruce King
Nov 22, 2011·Nitric Oxide : Biology and Chemistry·Sheng LiA Richard Whorton
May 1, 2012·Plant Physiology and Biochemistry : PPB·Martin C H Gruhlke, Alan J Slusarenko
May 31, 2012·Angewandte Chemie·Mauro Lo Conte, Kate S Carroll
Jul 10, 2012·Antioxidants & Redox Signaling·Bradley A MaronJoseph Loscalzo
Jul 12, 2012·Bioinformatics·Tzong-Yi LeeYu-Ju Chen
Nov 3, 2012·Methods : a Companion to Methods in Enzymology·Paschalis-Thomas DouliasHarry Ischiropoulos
Jan 3, 2013·Science Signaling·Paschalis-Thomas DouliasHarry Ischiropoulos
Jan 19, 2013·Circulation Research·Heaseung S ChungJennifer E Van Eyk

❮ Previous
Next ❯

Citations

Aug 30, 2016·Current Opinion in Biotechnology·Allison M RobertsDaniel K Nomura
Jan 10, 2017·ACS Chemical Biology·Marcus J C LongYimon Aye
Nov 15, 2017·Antioxidants & Redox Signaling·Masahiro Abo, Eranthie Weerapana
Dec 6, 2019·Chembiochem : a European Journal of Chemical Biology·Lisa J AlcockJustin M Chalker
Feb 8, 2020·Essays in Biochemistry·Leslie B PooleS Bruce King
Aug 16, 2017·Chembiochem : a European Journal of Chemical Biology·Yu-Hsuan KuoBrent R Martin
Dec 16, 2017·Chemical Society Reviews·Lisa J AlcockJustin M Chalker
Sep 5, 2018·Nature Chemical Biology·Salma AkterKate S Carroll
Sep 26, 2017·Chemical Communications : Chem Comm·Caihong ZhangMing Xian
Oct 22, 2020·Chemical Communications : Chem Comm·Fan Yang, Chu Wang
Oct 30, 2020·Antioxidants & Redox Signaling·Huan-Ling LaiElaine Lai-Han Leung
Dec 24, 2019·Accounts of Chemical Research·Yunlong Shi, Kate S Carroll
Mar 5, 2021·Chemical Reviews·Bauke AlbadaFloris van Delft
Jul 18, 2020·Cell Chemical Biology·Kazuki ShiraiwaItaru Hamachi
Apr 2, 2021·Plant Physiology·Patrick WillemsJingjing Huang
Jul 17, 2018·ACS Chemical Biology·Jonas DrechselStephan A Sieber
Nov 8, 2018·Journal of the American Chemical Society·Henry R Kilgore, Ronald T Raines
Jan 31, 2020·ACS Applied Materials & Interfaces·Julieta I PaezAránzazu Del Campo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.