HarpinPss encapsulation in chitosan nanoparticles for improved bioavailability and disease resistance in tomato

Carbohydrate Polymers
Sandhya Rani NadendlaAppa Rao Podile

Abstract

HarpinPss, an elicitor from Pseudomonas syringae pv. syringae, induces systemic acquired resistance in non-host plants, providing resistance to phytopathogens. Poor assimilation of harpinPss is a major constraint in foliar application as biopesticide. We, therefore, prepared harpinPss-loaded chitosan nanoparticles (H-CSNPs) to improve permeability and bio-availability of harpinPss in tomato. H-CSNPs showed high encapsulation efficiency (90%), improved stability (p < 0.01) and bioavailability of harpinPss (p < 0.01). Treatment with H-CSNPs resulted in sustained induction of peroxidase, phenylalanine ammonia lyase and decreased Rhizoctonia solani infection (p < 0.05). Transcripts of several genes involved in defense response were differentially expressed in harpinPss, CSNPs and H-CSNPs treatments. While, genes involved in jasmonic acid (JA) metabolism were up-regulated during harpinPss and H-CSNP spray treatments, indicating the role of JA pathway in triggering harpin-mediated defense responses. Furthermore, the entry of CSNPs into the cell and localization of harpinPss into chloroplast was tracked using rhodamine-labelled CSNPs encapsulated with GFP tagged harpinPss. The results of this study indicate use of H-CSNPs is effective...Continue Reading

Citations

May 11, 2019·Polymers·Massimo Malerba, Raffaella Cerana
Jan 5, 2020·Applied Microbiology and Biotechnology·Jyutika M RajwadeK M Paknikar
Nov 17, 2020·International Journal of Biological Macromolecules·Kamel R ShoueirMaged El-Kemary
Mar 21, 2020·International Journal of Biological Macromolecules·Muhammad MujtabaDepeng Wang
Jun 12, 2021·Frontiers in Chemistry·Mukesh MeenaPriyankaraj Sonigra

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