Has the code for protein translocation been broken?

Trends in Biochemical Sciences
Dalit Shental-BechorNir Ben-Tal

Abstract

Polypeptides chains are segregated by the translocon channel into secreted or membrane-inserted proteins. Recent reports claim that an in vivo system has been used to break the "amino acid code" used by translocons to make the determination of protein type (i.e. secreted or membrane-inserted). However, the experimental setup used in these studies could have confused the derivation of this code, in particular for polar amino acids. These residues are likely to undergo stabilizing interactions with other protein components in the experiment, shielding them from direct contact with the inhospitable membrane. Hence, it is our view that the "code" for protein translocation has not yet been deciphered and that further experiments are required for teasing apart the various energetic factors contributing to protein translocation.

References

Jun 22, 1989·Nature·M J Sternberg, W J Gullick
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D M EngelmanA Goldman
Jan 1, 1986·Journal of Cellular Biochemistry·D EisenbergA D McLachlan
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 18, 1999·Proteins·T J Stevens, I T Arkin
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Nov 21, 2002·Protein Science : a Publication of the Protein Society·Chien Peter ChenBurkhard Rost
Mar 12, 2003·Nature·Vanessa RutaRoderick MacKinnon
May 2, 2003·Nature·Youxing JiangRoderick MacKinnon
May 28, 2003·Nature Structural Biology·Christopher Miller
Sep 2, 2003·Current Opinion in Structural Biology·A Rachael Curran, Donald M Engelman
Dec 9, 2003·Journal of Molecular Biology·Salem FahamJames U Bowie
Jan 28, 2005·Nature·James U Bowie
Feb 1, 2005·Science·Tara HessaGunnar von Heijne
Feb 22, 2005·Current Opinion in Biotechnology·Horst Wallrabe, Ammasi Periasamy
Jul 9, 2005·Science·Stephen B LongRoderick Mackinnon
Jul 27, 2005·Current Opinion in Structural Biology·Stephen H White, Gunnar von Heijne
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Andrew R OsborneBert van den Berg
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·J Alfredo FreitesStephen H White

❮ Previous
Next ❯

Citations

Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sudha Dorairaj, Toby W Allen
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peter J Bond, Mark S P Sansom
Jul 20, 2007·The Journal of General Physiology·Benoit Roux
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna RychkovaArieh Warshel
Jun 27, 2012·The Journal of General Physiology·Keith M CallenbergMichael Grabe
Sep 21, 2011·Trends in Biochemical Sciences·Justin L MacCallum, D Peter Tieleman
Jul 11, 2006·Current Opinion in Structural Biology·Sarel J Fleishman, Nir Ben-Tal
Feb 21, 2016·Biochimica Et Biophysica Acta·James C Gumbart, Christophe Chipot
Aug 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Assaf ElazarSarel Jacob Fleishman
Aug 29, 2019·PLoS Computational Biology·Jonathan Yaacov WeinsteinSarel Jacob Fleishman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.