HAS2-AS1 is a novel LH/hCG target gene regulating HAS2 expression and enhancing cumulus cells migration

Journal of Ovarian Research
Y YungE Maman

Abstract

The cumulus expansion process is one of the LH mediated ovulatory processes. Hyaluronan synthase 2 (HAS2) regulates the synthesis of hyaluronic acid, the main component of the cumulus expansion process. Recently, the lncRNA HAS2 antisense RNA 1 (HAS2-AS1) was identified in our global transcriptome RNA-sequencing of novel ovulation associated genes. The role of HAS2-AS1 in HAS2 regulation w.as studied previously with contradictive results in different models but not in the ovary. Taken together the induction of HAS2-AS1 and the important role of HAS2 in the cumulus expansion process, we hypothesize that HAS2-AS1 regulate HAS2 expression and function in the ovary. Therefore we undertook to study the expression, regulation, and possible functional role of HAS2-AS1 in the human ovary. HAS2-AS1, located within the HAS2 gene that was highly regulated in our library. We found that HAS2-AS1 express mainly in cumulus cells (CCs). Furthermore, HAS2-AS1 showed low expression in immature CCs and a significant increase expression in mature CCs. Functional studies reveal that inhibition of HAS2-AS1 by siRNA caused decrease expression of HAS2. Furthermore, inhibition of HAS2-AS1 by siRNA results in decrease migration of granulosa cells. Our r...Continue Reading

References

Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·H HizakiA Ichikawa
Jan 24, 2002·The Journal of Biological Chemistry·Haikun ShiHaim Garty
Jun 20, 2002·Reproduction in Domestic Animals = Zuchthygiene·Ann Van SoomA de Kruif
Sep 17, 2002·European Journal of Biochemistry·Haikun ShiHaim Garty
Jun 29, 2004·International Review of Cytology·Masaki Yokoo, Eimei Sato
Apr 20, 2005·Molecular and Cellular Endocrinology·Joanne S Richards
Apr 22, 2005·The Journal of Biological Chemistry·Hsu Chao, Andrew P Spicer
Sep 30, 2006·Reproductive Biomedicine Online·Ariel HourvitzJehoshua Dor
Jan 24, 2007·Human Reproduction Update·Darryl L Russell, Rebecca L Robker
Apr 7, 2010·The Journal of Clinical Investigation·Joanne S Richards, Stephanie A Pangas
Feb 3, 2011·Progress in Molecular and Subcellular Biology·Riki Kurokawa
Nov 23, 2011·Trends in Plant Science·Eun-Deok Kim, Sibum Sung
May 23, 2012·DNA and Cell Biology·Biao Yan, Zhenhua Wang
May 31, 2014·Molecular Human Reproduction·Y YungA Hourvitz
Dec 17, 2014·Journal of Ovarian Research·Alon KedemAriel Hourvitz
Sep 6, 2017·Trends in Genetics : TIG·Allison Jandura, Henry M Krause
Jan 1, 2016·Fiziolohichnyĭ zhurnal·E A ShepelR I Yanchii

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Citations

Oct 23, 2019·Molecular Reproduction and Development·Yu ZhangMeijia Zhang
Jun 7, 2020·Journal of Ovarian Research·Jiajie TuZhiying Yu
Jul 19, 2019·Seminars in Cancer Biology·Ilaria CaonAlberto Passi
Aug 7, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Timpika ThepsuwanPonsit Sathapondecha

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Methods Mentioned

BETA
contraception
PCR

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