PMID: 9165105Apr 25, 1997Paper

Hatching enzyme from the sea-squirt Ciona intestinalis: purification and properties

Biochimica Et Biophysica Acta
A D'AnielloS Scippa

Abstract

We have purified a 34 kDa hatching enzyme from the water in which the embryos of the sea-squirt Ciona intestinalis hatch. This enzyme was obtained in homogeneous form as judged from SDS-PAGE and HPLC gel filtration. The enzyme possesses proteolytic activity and is able to digest the chorion of the egg of C. intestinalis. It is a metalloproteinase and contains one atom of Zn per molecule. The optimum pH is 8.5. The enzyme shows hydrolytic activity towards the -CO-NH- bonds, which are hydrolyzed by the members of the serine proteinase family. It has a trypsin-like activity in that it cuts the bond of Arg and Lys at P1 position of the scissile bond -P1-P1', but it differs from trypsin insofar as it hydrolyzes the peptide bond on either side of Arg and Lys. The purified enzyme is inhibited by the common metal-chelators and by the classical trypsin proteinase inhibitors. The apparent K(m) values at 37 degrees C and pH 8.5 toward tosyl-Gly-Pro-Arg-NHNap, tosyl-Gly-Pro-Lys-NHNap and Bz-Arg-Gly-Arg-NHNap were 0.125, 0.5 and 2.5 mM, respectively. The results obtained in this study suggest that the hatching enzyme from C. intestinalis exhibits both trypsin-like activity and metalloproteinase activity.

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Mar 20, 1947·The Journal of General Physiology·M Kunitz

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Citations

Jan 12, 2013·Applied Biochemistry and Biotechnology·Zhi Jiang Li, Sang Moo Kim
Sep 28, 1998·Biochimica Et Biophysica Acta·Y Kitamura, C Katagiri
May 13, 2004·Molecular and Biochemical Parasitology·Elena S KovalevaDavid J Chitwood
Apr 18, 2016·International Journal of Biological Macromolecules·Ahmad HomaeiReyhaneh Sariri
Dec 23, 2014·Natural Product Research·Chanutchamon Sutthiwanjampa, Sang Moo Kim
Mar 1, 2006·Fish Physiology and Biochemistry·Zhen-Ping ShiLing-Ling Yang
Feb 1, 2019·Scientific Reports·Shiori NakazawaHitoshi Sawada
Feb 2, 2010·Acta Biochimica Et Biophysica Sinica·Tingjun FanRishan Cong
May 23, 2006·Archives of Biochemistry and Biophysics·Bing-Jun LiZhen-Ping Shi

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