Hazelnut (Corylus avellana) vicilin Cor a 11: molecular characterization of a glycoprotein and its allergenic activity

The Biochemical Journal
Iris LauerStephan Scheurer

Abstract

In Europe, hazelnuts (Corylus avellana) are a frequent cause of food allergies. Several important hazelnut allergens have been previously identified and characterized. Specific N-glycans are known to induce strong IgE responses of uncertain clinical relevance, but so far the allergenic potential of glycoproteins from hazelnut has not been investigated. The aim of the study was the molecular characterization of the glycosylated vicilin Cor a 11 from hazelnut and the analysis of its allergenic activity. Although MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS showed that one of two potential glycosylation sites of Cor a 11 was glycosylated, CD spectroscopy indicated that recombinant and natural Cor a 11 share similar secondary structures. Thus to analyse the impact of the glycan residues of Cor a 11 on IgE binding, the allergenic activity of natural glycosylated Cor a 11 and recombinant Cor a 11 was compared. In addition, the IgE sensitization pattern to recombinant Cor a 11, Cor a 1, Cor a 2 and Cor a 8 of 65 hazelnut allergic patients was determined in vitro. The prevalence of IgE reactivity to hazelnut vicilin Cor a 11 was below 50%. Basophil histamine-release assays were used to determine the allerge...Continue Reading

References

Aug 1, 1994·International Archives of Allergy and Immunology·S ViethsA Petersen
Aug 31, 1996·BMJ : British Medical Journal·S M TariqD W Hide
Oct 26, 1999·International Archives of Allergy and Immunology·K FötischS Vieths
Jan 30, 2002·International Archives of Allergy and Immunology·I Kleine BuddeR C Aalberse
Mar 19, 2002·The Journal of Allergy and Clinical Immunology·Elide A PastorelloAmedeo Conti
Jul 12, 2002·The Journal of Allergy and Clinical Immunology·Fang WangKenneth H Roux
Sep 5, 2002·The Journal of Allergy and Clinical Immunology·Kirsten BeyerHugh A Sampson
Apr 22, 2003·The Journal of Allergy and Clinical Immunology·Kay FoetischStefan Vieths
Jul 4, 2003·Current Opinion in Allergy and Clinical Immunology·Kirsten Beyer
Aug 14, 2003·International Archives of Allergy and Immunology·Kenneth H RouxShridhar K Sathe
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Merima BublinKarin Hoffmann-Sommergruber
Jan 10, 2004·The Journal of Allergy and Clinical Immunology·Frauke SchockerWolf-Meinhard Becker

❮ Previous
Next ❯

Citations

Jan 22, 2010·Analytical and Bioanalytical Chemistry·Eric A E Garber, Jesse Perry
Jul 8, 2011·Analytical and Bioanalytical Chemistry·Parisa AnsariSabine Baumgartner
Jul 16, 2010·Current Allergy and Asthma Reports·Kirsten Skamstrup Hansen, Lars K Poulsen
May 14, 2011·Journal of Agricultural and Food Chemistry·Malgorzata IwanHarry J Wichers
Sep 18, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M ArlorioA Martelli
May 24, 2012·Journal of Clinical Bioinformatics·Elena LópezJesús F Crespo
Jul 7, 2012·PloS One·Stefano AlessandriKarin Hoffmann-Sommergruber
Dec 22, 2011·Journal of Allergy·Pedro A Alvarez, Joyce I Boye
Feb 21, 2014·Current Allergy and Asthma Reports·Merima Bublin, Heimo Breiteneder
May 15, 2013·Journal of Proteomics·Chiara NitridePasquale Ferranti
Dec 2, 2011·Annual Review of Food Science and Technology·Mary Rose G Tandang-SilvasNobuyuki Maruyama
Apr 7, 2009·The Journal of Allergy and Clinical Immunology·Kirsten Skamstrup HansenLars K Poulsen
Nov 27, 2007·The Journal of Allergy and Clinical Immunology·Annebeth E FlintermanSuzanne G Pasmans
Oct 22, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B BohleC Ebner
Jan 11, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S KaulA Hoffmann
May 23, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P RupaY Mine
Nov 14, 2008·Molecular Nutrition & Food Research·Neil M RigbyE N Clare Mills
May 16, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L N WillisonK H Roux
Aug 8, 2008·Molecular Nutrition & Food Research·Iris LauerStephan Scheurer
May 17, 2011·Allergy·R C Aalberse
Feb 24, 2011·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·K J De KnopD G Ebo
Jan 13, 2006·Biochimica Et Biophysica Acta·Daniel KolarichElumalai Sunderasan
Oct 19, 2005·Current Opinion in Immunology·Birgit Linhart, Rudolf Valenta
Apr 18, 2016·Molecular Aspects of Medicine·Ian LokeMorten Thaysen-Andersen
Mar 17, 2015·The Journal of Allergy and Clinical Immunology·Mareen R DatemaRonald van Ree
Apr 16, 2013·The Journal of Allergy and Clinical Immunology·Laury J N MasthoffSuzanne G M A Pasmans
Mar 10, 2015·Critical Reviews in Food Science and Nutrition·Joana CostaMaria Beatriz P P Oliveira
Feb 6, 2015·Asia Pacific Allergy·Jian Yi SohBee Wah Lee
Nov 17, 2005·Molecular Nutrition & Food Research·Jaap H AkkerdaasRonald van Ree
May 4, 2006·Current Opinion in Allergy and Clinical Immunology·Jonas LidholmStefan Vieths
Oct 13, 2006·International Archives of Allergy and Immunology·Friedrich Altmann
Jun 19, 2008·Current Opinion in Allergy and Clinical Immunology·Annebeth E FlintermanSuzanne G Pasmans
Apr 10, 2020·Journal of the Science of Food and Agriculture·Yuzhu Zhang, Tengchuan Jin
Jun 10, 2020·Critical Reviews in Biotechnology·Jeppe Madura LarsenKatrine Lindholm Bøgh
Sep 3, 2020·Clinical Reviews in Allergy & Immunology·Joana CostaThomas Holzhauser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.