HB-EGF expression as a potential biomarker of acquired middle ear cholesteatoma

Acta Oto-laryngologica
Shumin XieWei Liu

Abstract

The heparin-binding epidermal growth factor-like growth factor (HB-EGF) plays an essential role in the development and invasiveness of cholesteatoma. This study may help to realize the molecular mechanisms underlying the pathogenesis of cholesteatoma and make HB-EGF a promising target for drug intervention of cholesteatoma. To detect HB-EGF expression in human surgical specimens of acquired middle ear cholesteatoma and analyze its functional role as a regulator of epithelial keratinocytes hyperproliferation. A total of 34 patients who underwent surgical treatment for middle ear cholesteatoma were recruited in the study. The mRNA and protein expression of HB-EGF in middle ear cholesteatoma tissues and normal postauricular skin tissues was investigated by real-time quantitative reverse-transcription-polymerase chain reaction (RT-qPCR), immunohistochemical staining, and western blot. The correlation between bone resorption degree and HB-EGF expression was also analyzed. On average, compared with normal postauricular skin, expression of HB-EGF mRNA in the cholesteatoma epithelium was significantly elevated 2.41-fold by RT-qPCR, and HB-EGF protein significantly upregulated 2.32-fold by western blot. Positive HB-EGF immunostaining ob...Continue Reading

References

Dec 18, 2009·American Journal of Otolaryngology·Jerzy KuczkowskiEwa Iżycka-Świeszewska
Mar 7, 2013·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Wei LiuDinghua Xie
Nov 22, 2013·Acta Oto-laryngologica·Mette Bendixen ThorupTherese Ovesen
Aug 1, 2015·World Journal of Gastroenterology : WJG·Woo Chul ChungJung Hwan Oh
Dec 10, 2015·Cancer Cell International·Danielle L BourgeoisPamela K Kreeger
Mar 15, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ryo IwamotoEisuke Mekada

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