HBV secretion is regulated through the activation of endocytic and autophagic compartments mediated by Rab7 stimulation

Journal of Cell Science
Jun InoueMark A McNiven

Abstract

The cellular mechanisms by which hepatitis B virus (HBV) is assembled and exported are largely undefined. Recently, it has been suggested that these steps require the multivesicular body (MVB) and the autophagic machinery. However, the mechanisms by which HBV might regulate these compartments are unclear. In this study, we have found that by activating Rab7a, HBV alters its own secretion by inducing dramatic changes in the morphology of MVB and autophagic compartments. These changes are characterized by the formation of numerous tubules that are dependent upon the increase in Rab7 activity observed in the HBV-expressing HepG2.2.15 cells compared to HepG2 cells. Interestingly, transfection-based expression of the five individual viral proteins indicated that the precore protein, which is a precursor of HBeAg, was largely responsible for the increased Rab7 activity. Finally, small interfering RNA (siRNA)-mediated depletion of Rab7 significantly increased the secretion of virions, suggesting that reduced delivery of the virus to the lysosome facilitates viral secretion. These findings provide novel evidence indicating that HBV can regulate its own secretion through an activation of the endo-lysosomal and autophagic pathway mediate...Continue Reading

References

May 16, 1998·The Journal of Cell Biology·J R HenleyM A McNiven
Feb 26, 2000·Molecular Biology of the Cell·C BucciB van Deurs
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David Milich, T Jake Liang
Sep 2, 2004·Journal of Cell Science·Stefanie JägerEeva-Liisa Eskelinen
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Atsushi OzasaMasashi Mizokami
Jan 9, 2007·World Journal of Gastroenterology : WJG·Volker Bruss
Jun 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tokiko WatanabePaul Ahlquist
Nov 15, 2008·Cell Death and Differentiation·C M Fader, M I Colombo
Mar 7, 2009·The Journal of Biological Chemistry·Phillip A Vanlandingham, Brian P Ceresa
Apr 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T Jake Liang
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Harald Stenmark
Nov 17, 2009·The Journal of Biological Chemistry·Wentao GaoXiao-Ming Yin
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Donna SirJing-Hsiung James Ou
Sep 21, 2010·Cellular Signalling·Tuanlao WangWanjin Hong
Dec 12, 2012·Biochimica Et Biophysica Acta·Juha M T HyttinenKai Kaarniranta
Oct 23, 2013·The Journal of Cell Biology·Ryan J SchulzeMark A McNiven
Mar 13, 2014·PloS One·Jens T Stieler, Reinhild Prange

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Citations

Jan 19, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kenichi MorikawaNaoya Sakamoto
Aug 23, 2016·Cells·Flora Guerra, Cecilia Bucci
Jun 27, 2017·Nature Reviews. Microbiology·Nancy Raab-Traub, Dirk P Dittmer
Dec 14, 2017·Essays in Biochemistry·Aurore Claude-TaupinVojo Deretic
Jan 14, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun InoueMark A McNiven
Sep 5, 2019·Viral Immunology·Asghar AbdoliAmitis Ramezani
Jul 26, 2018·Oncotarget·Yutaka NaitoYasuhito Tanaka
Feb 12, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yong LinMengji Lu
Jun 16, 2019·The Journal of Cell Biology·Ryan J SchulzeMark A McNiven
Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Akriti PrasharMaximiliano G Gutierrez
Aug 29, 2020·Archives of Gynecology and Obstetrics·Peng ZhaoXiaoxia Bai
Dec 5, 2018·Frontiers in Cell and Developmental Biology·Lisa Zeyen, Reinhild Prange
Dec 22, 2020·Antiviral Research·Yuzy FauzyahYoshiharu Matsuura

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