HBx triggers either cellular senescence or cell proliferation depending on cellular phenotype

Journal of Viral Hepatitis
M E IdrissiE Wattel

Abstract

Replicative senescence is a hallmark of chronic liver diseases including chronic hepatitis B virus (HBV) infection, whereas HBV-encoded oncoproteins HBx and preS2 have been found to overcome senescence. HBx possesses a C-terminal truncation mainly in hepatocellular carcinomas but also in noncancerous liver tissues. Here, by cell counting, BrdU incorporation, MTT proliferation assay, cell cycle analysis, SA-βgal staining and Western blotting in primary and malignant cells, we investigated the effect of HBx C-terminal mutants on cellular senescence. HBx C-terminal mutants were found to trigger cellular senescence in primary MRC5 cells, and malignant liver cells Huh7, and SK-Hep1. In contrast, these mutants promoted the proliferation of HepG2 malignant liver cells. The pro-senescent effect of HBx relied on an increased p16(INK4a) and p21(Waf1/Cip1) expression, and a decreased phosphorylation of Rb. Together, these results suggest that the two main variants of HBx present in HBV-infected liver possess opposite effects on cellular senescence that depend on the phenotype of infected cells.

References

May 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H C HsuS Y Peng
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W H CaselmannR Koshy
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·X W WangC C Harris
Feb 10, 2000·Annals of the New York Academy of Sciences·G CarrubaL Castagnetta
Nov 20, 2002·Oncogene·Tolga Cagatay, Mehmet Ozturk
Jan 21, 2006·World Journal of Gastroenterology : WJG·Gerald C KimbiMichael C Kew
Apr 22, 2006·Aging Cell·Bo Yun LeeEun Seong Hwang
Jul 31, 2007·Cell·Manuel ColladoManuel Serrano
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Jul 8, 2008·Biochemical and Biophysical Research Communications·Xiaohong LiuMinghua Zhu
Aug 14, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ning-Fang MaXin-Yuan Guan
Dec 17, 2008·Cancer Letters·Mehmet OzturkHaluk Yuzugullu
May 8, 2009·Cellular and Molecular Life Sciences : CMLS·Eun Seong HwangHyun Tae Kang
May 28, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiaoying ZhangShuhan Sun
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Haeryoung KimYoung Nyun Park
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jiejie XuJianxin Gu
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Michael C Kew
Dec 1, 2009·Viruses·Philippe Merle, Christian Trepo
May 29, 2012·Nature Genetics·Wing-Kin SungJohn M Luk
Jul 24, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karen M F SzeIrene O L Ng
Jan 25, 2013·Nature Reviews. Cancer·Alla ArzumanyanMark A Feitelson
Sep 12, 2013·Journal of Experimental & Clinical Cancer Research : CR·Manale El IdrissiEric Wattel

❮ Previous
Next ❯

Citations

Dec 30, 2016·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Guo-Wen LiKai Qing
Sep 7, 2017·Scientific Reports·Satoru HagiwaraMasatoshi Kudo
Sep 6, 2020·Liver International : Official Journal of the International Association for the Study of the Liver·Nikolaos D KarakousisGeorge Papatheodoridis
Dec 16, 2020·Biology·Rocío SeoaneCarmen Rivas
Dec 30, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Adonis SferaJose Campo Maldonado

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Related Papers

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
T PollicinoM A Buendia
European Journal of Obstetrics, Gynecology, and Reproductive Biology
Feng YeLinshu Zhang
© 2021 Meta ULC. All rights reserved