HCN1 channels constrain DHPG-induced LTD at hippocampal Schaffer collateral-CA1 synapses

Learning & Memory
Tursonjan TokayTimo Kirschstein

Abstract

HCN channels play a fundamental role in determining resting membrane potential and regulating synaptic function. Here, we investigated the involvement of HCN channels in basal synaptic transmission and long-term depression (LTD) at the Schaffer collateral-CA1 synapse. Bath application of the HCN channel blocker ZD7288 (10 microM) caused a significant increase in synaptic transmission that was due to an enhancement in AMPA receptor-mediated excitatory postsynaptic potentials. This enhancement was accompanied by a significant decrease in the paired-pulse ratio (PPR), suggesting a presynaptic mechanism. Experiments with the irreversible use-dependent NMDA receptor blocker MK-801 showed that ZD7288 led to an increase in glutamate release probability. LTD induced by brief application of (RS)-3,5-dihydroxyphenylglycine (DHPG, 100 microM, 10 min) was significantly enhanced when HCN channels were blocked by ZD7288 (10 microM) prior to DHPG application. Moreover, the concomitant increase in PPR after DHPG-induced LTD was significantly larger than without ZD7288 bath application. Conversely, ZD7288 application after DHPG washout did not alter DHPG-LTD. A significant enhancement of DHPG-LTD was also observed in HCN1-deficient mice as comp...Continue Reading

References

Jan 31, 1998·Pflügers Archiv : European journal of physiology·S Gasparini, D DiFrancesco
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Vivien Chevaleyre, Pablo E Castillo
Mar 3, 2004·The Journal of Comparative Neurology·Takuya Notomi, Ryuichi Shigemoto
Mar 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peter R MoultGraham L Collingridge
May 13, 2006·The Journal of Physiology·Yexica ApontePeter Jonas
Apr 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Roland A BenderTallie Z Baram

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