PMID: 9630649Jun 19, 1998Paper

HCO3(-)-dependent ion transport systems and intracellular pH regulation in colonocytes from the chick

Biochimica Et Biophysica Acta
M L Calonge, A Ilundáin

Abstract

The current study examines the presence of the Na+/HCO3- cotransporter and of the Cl-/HCO3- exchanger in chicken colonocytes and their role in cytosolic pH (pHi) homeostasis. pHi was measured with 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) at 25 degreesC. Basal pHi was 7.16 in HEPES-buffered solutions and 7.06 in those buffered with HCO3-. Removal of external Cl- increased pHi and Cl- reinstatement brought the pHi towards resting values. These Cl--induced pHi changes were Na+-independent, inhibited by H2-DIDS and faster in the presence than in the absence of HCO3-. Cells recovered from alkaline loads by a mechanism that was Cl--dependent, Na+-independent and inhibited by H2-DIDS. This rate of Cl--dependent cell acidification decreased as the pHi decreased, with a Hill coefficient value close to 4. Removal of external Na+ decreased pHi and readdition of Na+ brought pHi towards the control values. The rate of the Na+-induced changes was not modified by the presence of HCO3- and was prevented by EIPA and unaffected by H2-DIDS. In the presence of EIPA cells partially recovered from a moderate acid load only when both Na+ and HCO3- were present. The EIPA resistant Na+- and bicarbonate-dependent pHi recovery was inhibited...Continue Reading

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Citations

Jun 19, 2001·Biochimica Et Biophysica Acta·I VaneckováJ Pácha
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Dec 17, 2004·American Journal of Physiology. Cell Physiology·Elizabeth A CowleyNicholas P Illsley
May 29, 2018·World's Poultry Science Journal·Meghali Nighot, Prashant Nighot

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