PMID: 41100Oct 5, 1979

HCO3-/Cl- exchange across the human erythrocyte membrane: effects of pH and temperature

The Journal of Membrane Biology
A L Obaid, E D Crandall

Abstract

Changes in extracellular pH (PH0) in red cell suspensions were monitored in a stopped-flow rapid reaction apparatus under conditions where dpH0/dt was determined by the rate of HCO3-/Cl- exchange across the membrane. Experiments were performed at 5 degrees C less than T less than 40 degrees C using either untreated cells or cells exposed to 0.11 mM SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid). Although SITS exposure reduced the rate of exchange by 90%, both untreated and SITS-treated cells are similarly affected by changes in pH0 and temperature. The rate of HCO3-/Cl- exchange exhibits a minimum at about pH0 5 and a maximum at about pH0 7.4 at all temperatures. A transition temperature of 17 degrees C was observed in the Arrhenius relationship for all pH0. The activation energies (Ea) in kcal/mol are 19.6 below and 11.7 above 17 degrees C for 5 less than pH0 less than 8. These findings, similar to those reported for Cl- self-exchange, suggest that: (i) a change in the rate-limiting step for HCO3-/Cl- exchange occurs at 17 degrees C, possibly due to an altered interaction between the transport pathway and membrane lipids; (ii) the carrier system can be titrated by either H+ or SITS from the outside of the memb...Continue Reading

References

Jan 1, 1980·The Journal of Membrane Biology·A L ObaidE D Crandall
Jan 1, 1982·The Journal of Membrane Biology·E D CrandallA Bidani
May 23, 1980·The Journal of Membrane Biology·A M Critz, E D Crandall
Aug 19, 1983·Neuroscience Letters·H Kettenmann, R K Orkand
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N B He, S W Hui
Aug 20, 1981·Biochimica Et Biophysica Acta·E I ChowP K Tseng
Oct 25, 1991·Brain Research·Q R Smith, C E Johanson
Jun 27, 1983·FEBS Letters·V A Gregg, R A Reithmeier

Citations

Jun 1, 1975·The Journal of General Physiology·R B GunnD C Tosteson
Nov 1, 1976·The Journal of Physiology·J Funder, J O Wieth
Jan 28, 1977·The Journal of Membrane Biology·K F SchnellA Schöppe-Fredenburg
Sep 1, 1977·The Journal of General Physiology·J Brahm
Dec 8, 1978·The Journal of Membrane Biology·W P Dubinsky, E Racker
Aug 1, 1975·The Journal of Physiology·M Dalmark
Feb 1, 1978·The Journal of Physiology·A Lambert, A G Lowe
Dec 1, 1976·The Journal of General Physiology·E I ChowR E Forster
May 1, 1975·Quarterly Reviews of Biophysics·D Chapman
Feb 1, 1973·The Journal of General Physiology·R B GunnJ O Wieth
Oct 1, 1973·Journal of Cellular Physiology·L LackoP Geck
Mar 29, 1974·Biochimica Et Biophysica Acta·F J Blatt
Apr 1, 1970·Die Naturwissenschaften·B Deuticke
Jan 1, 1971·Pflügers Archiv : European journal of physiology·G Gros, W Moll
Dec 1, 1970·Biochimica Et Biophysica Acta·M Dalmark, J O Wieth
May 1, 1967·The Journal of General Physiology·R I Sha'afiA K Solomon
Mar 20, 1942·The Journal of General Physiology·M H Jacobs, D R Stewart
Dec 1, 1972·The Journal of Membrane Biology·A L ObaidP J Garrahan

Related Concepts

Chloride Ion Level
AT 17
Extracellular
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, Disodium Salt
Carbonic Acid Ions
Bicarbonate Measurement
Erythrocyte Membrane
Bicarbonates
Red Cell Ghost
Transport Pathway

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