HCS-Analyzer: open source software for high-content screening data correction and analysis

Bioinformatics
Arnaud Ogier, Thierry Dorval

Abstract

High-throughput screening is a powerful technology principally used by pharmaceutical industries allowing the identification of molecules of interest within large libraries. Originally target based, cellular assays provide a way to test compounds (or other biological material such as small interfering RNA) in a more physiologically realistic in vitro environment. High-content screening (HCS) platforms are now available at lower cost, giving the opportunity for universities or research institutes to access those technologies for research purposes. However, the amount of information extracted from each experiment is multiplexed and hence difficult to handle. In such context, there is an important need for an easy-to-use, but still powerful software able to manage multidimensional screening data by performing adapted quality control and classification. HCS-analyzer includes: a user-friendly interface specifically dedicated to HCS readouts, an automated approach to identify systematic errors potentially occurring during screening and a set of tools to classify, cluster and identify phenotypes of interest among large and multivariate data. The application, the C# .Net source code, as well as detailed documentation, are freely availa...Continue Reading

References

Jan 9, 2004·Journal of Biomolecular Screening·Christine BrideauAndy Liaw
Aug 13, 2010·Bioinformatics·Phoenix Kwan, Amanda Birmingham
Jan 21, 2011·BMC Bioinformatics·Plamen DragievVladimir Makarenkov
Nov 29, 2011·Bioinformatics·Jean-Philippe CarralotThierry Dorval

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Citations

Jun 24, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M J VitaloneM M Sarwal
Mar 15, 2016·Expert Opinion on Drug Discovery·Ivan Fraietta, Fabio Gasparri
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Mar 19, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marc Thilo Figge
Nov 12, 2016·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Orsolya BiróJános Rigó
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Oct 30, 2016·Briefings in Bioinformatics·Shardul ParicharakGerard J P van Westen
Jun 21, 2020·Drug Discovery Today·Sean LinKamyar Hadian

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