HCS road: an enterprise system for integrated HCS data management and analysis

Journal of Biomolecular Screening
Donald JacksonJames Gill

Abstract

The effective analysis and interpretation of high-content screening (HCS) data requires joining results to information on experimental treatments and controls, normalizing data, and selecting hits or fitting concentration-response curves. HCS data have unique requirements that are not supported by traditional high-throughput screening databases, including the ability to designate separate positive and negative controls for different measurements in multiplexed assays; the ability to capture information on the cell lines, fluorescent reagents, and treatments in each assay; the ability to store and use individual-cell and image data; and the ability to support HCS readers and software from multiple vendors along with third-party image analysis tools. To address these requirements, the authors developed an enterprise system for the storage and processing of HCS images and results. This system, HCS Road, supports target identification, lead discovery, lead evaluation, and lead profiling activities. A dedicated client supports experimental design, data review, and core analyses and displays images together with results for assay development, hit assessment, and troubleshooting. Data can be exported to third-party applications for fu...Continue Reading

References

Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Nov 13, 2004·Science·Zachary E PerlmanSteven J Altschuler
Feb 9, 2006·Nature Biotechnology·Nathalie MaloRobert Nadon
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Nov 9, 2007·Journal of Biomolecular Screening·Michele AglerTaosheng Chen
Mar 14, 2008·Combinatorial Chemistry & High Throughput Screening·Xuefeng Bruce Ling
Nov 13, 2008·Molecular Cancer Therapeutics·Petra Ross-MacdonaldDonald Jackson
May 7, 2009·Annual Review of Biophysics·Jason R SwedlowUNKNOWN OME Consortium
Jun 18, 2009·Combinatorial Chemistry & High Throughput Screening·Mark A Collins
Aug 1, 2009·Journal of Biomolecular Screening·Mike PalmerGeorg C Terstappen
Nov 26, 2009·Combinatorial Chemistry & High Throughput Screening·Joseph M Zock

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Citations

Jul 17, 2012·Journal of Laboratory Automation·Constantin RaduHakim Djaballah
Jan 12, 2012·Journal of Biomolecular Screening·Frans CornelissenEmmanuel Gustin
Jun 3, 2020·SLAS Discovery·Judith Wardwell-SwansonOlivier Frey

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Methods Mentioned

BETA
Assay
transgenic

Software Mentioned

TIBCO Spotfire workflow
TIBCO Spotfire
Evotec ImageView
Image Magick
InCell
HCS Data Explorer
SQL
HCS Road
CellProfiler
Accelerys PipelinePilot

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