HCV genotype-3a T cell immunity: specificity, function and impact of therapy.

Gut
Isla HumphreysEleanor Barnes

Abstract

Hepatitis C virus (HCV) genotype-3a infection is now the dominant strain in South Asia and the UK. Characteristic features include a favourable response to therapy; the reasons for this are unknown but may include distinct genotype-3a-specific T cell immunity. In contrast to genotype-1 infection, T cell immunity to this subtype is poorly defined. The aims of the study were to (1) define the frequency, specificity and cross-reactivity of T cell immunity across the whole viral genome in genotype-3a infection and (2) assess the impact of interferon (IFN)-α/ribavirin on T cell immunity. T cell responses in chronic and resolved HCV genotype-3a were analysed in comparison with genotype-1 infection (total n=85) using specific peptide panels in IFN-γ ELISpot assays. T cell responses were followed longitudinally in a subset of genotype-3a infected patients receiving therapy. Responses were further defined by CD4 and CD8 subset analysis, sequencing of autologous virus and cross-reactivity of genotype-3a with genotype-1a/-1b antigens. CD8 T cell responses commonly targeted the non-structural (NS) proteins in chronic genotype-3a infection whereas in genotype-1 infection CD4 responses targeting HCV core predominated (p=0.0183). Resolved inf...Continue Reading

Citations

Aug 21, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Anna PasettoMargaret Chen
Nov 28, 2012·Current HIV/AIDS Reports·Rebecca R Terilli, Andrea L Cox
Apr 20, 2016·BMC Infectious Diseases·Amina GulIjaz Ali
Oct 17, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christabel KellyEleanor Barnes
Feb 14, 2018·Journal of Virology·Danushka K WijesundaraBranka Grubor-Bauk
Feb 6, 2018·Journal of Viral Hepatitis·Hendrik LuxenburgerChristoph Neumann-Haefelin
Apr 3, 2015·World Journal of Gastroenterology : WJG·Juan-Ramón LarrubiaEduardo Sanz-de-Villalobos
Feb 2, 2019·World Journal of Hepatology·Luís Henrique Bezerra Cavalcanti SetteNorma Lucena-Silva

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Datasets Mentioned

BETA
GQ356217
D28917
AF009606

Methods Mentioned

BETA
PCR

Software Mentioned

ClustalX
FlowJo
MEGA5

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