Abstract
Recent advances in interferon-free treatment could lead to the eradication of hepatitis C virus (HCV) from patients infected with HCV. One of the direct-acting anti-viral agents, HCV NS5A inhibitor, is available for these combination therapies. However, naturally occurring resistance-associated variants (RAVs) to HCV NS5A inhibitors in treatment-naïve patients chronically infected with HCV genotype 1b are still unknown. We performed ultra-deep sequencing and analysed previously reported RAVs in a total 132 HCV genotype 1b-infected Japanese patients who had never used HCV NS5A inhibitors. We also performed direct-sequencing by Sanger method in consecutively selected 50 of the total 132 samples, and the differences between the results of the two methods were compared. In the comparison of the variant frequencies of ultra-deep sequencing with RAVs of direct-sequencing by Sanger method in 50 patients, we identified 32 RAVs by direct-sequencing with the Sanger method; minimum variant frequency was shown by ultra-deep sequencing to be 9%. A total of 110 RAVs were identified only by ultra-deep sequencing. In the samples from all 132 patients, L31W (2.3%), L31V (49.2%), L31F (41.7%), L31M (1.5%), L31I (5.3%), L31S (2.0%), L31P (3.0%) a...Continue Reading
References
Sep 26, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M Di Bisceglie
Jan 20, 2005·Annals of Internal Medicine·Yasushi ShiratoriUNKNOWN Tokyo-Chiba Hepatitis Research Group
Feb 15, 2007·Reviews in Medical Virology·Koichi Watashi, Kunitada Shimotohno
Jun 10, 2008·Journal of Hepatology·Sylvie Deuffic-BurbanPhilippe Mathurin
Mar 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·V SchregelN Tautz
Jun 30, 2010·Antimicrobial Agents and Chemotherapy·Robert A FridellMin Gao
Oct 19, 2010·Lancet·Edward J GanePatrick F Smith
Nov 11, 2010·Hepatology International·Tatsuo KandaOsamu Yokosuka
Jan 20, 2012·The New England Journal of Medicine·Anna S LokClaudio Pasquinelli
Jun 5, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Fumitaka SuzukiHiromitsu Kumada
Jul 26, 2012·BMC Genomics·Michael A QuailYong Gu
Nov 16, 2012·Journal of Virology·Doug J BartelsTara L Kieffer
Nov 28, 2012·Journal of Hepatology·Yoshiyuki SuzukiHiromitsu Kumada
Apr 9, 2013·Nature Biotechnology·Sebastian JünemannDag Harmsen
Jul 3, 2013·Nature Reviews. Gastroenterology & Hepatology·Behzad HajarizadehGregory J Dore
Sep 27, 2013·PloS One·Shuang WuOsamu Yokosuka
Jan 17, 2014·The New England Journal of Medicine·Mark S SulkowskiUNKNOWN AI444040 Study Group
Mar 8, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hiromitsu KumadaEric Hughes
Mar 13, 2014·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Mika MiuraNobuyuki Enomoto
Mar 25, 2014·World Journal of Gastroenterology : WJG·Shingo NakamotoOsamu Yokosuka
Apr 12, 2014·The New England Journal of Medicine·Stefan ZeuzemBarry Bernstein
Apr 12, 2014·The New England Journal of Medicine·Kris V KowdleyUNKNOWN ION-3 Investigators
Apr 12, 2014·The New England Journal of Medicine·Jordan J FeldBarry Bernstein
Apr 15, 2014·The New England Journal of Medicine·Fred PoordadBarry Bernstein
Apr 15, 2014·The New England Journal of Medicine·Nezam AfdhalUNKNOWN ION-2 Investigators
Apr 15, 2014·The New England Journal of Medicine·Nezam AfdhalUNKNOWN ION-1 Investigators
May 6, 2014·The New England Journal of Medicine·Peter FerenciUNKNOWN PEARL-IV Study
May 6, 2014·The New England Journal of Medicine·Stefan ZeuzemUNKNOWN VALENCE Investigators
Aug 1, 2014·Lancet·Michael MannsUNKNOWN HALLMARK-DUAL Study Team
Aug 12, 2014·Antiviral Therapy·Tanya L ApplegateGail V Matthews
Citations
Mar 10, 2015·International Journal of Molecular Sciences·Tatsuo KandaMasao Omata
Sep 22, 2015·Viruses·Lize CuypersKristof Theys
May 1, 2016·Hepatology International·Masao OmataShiv Kumar Sarin
Jun 11, 2016·Hepatology International·Tatsuo Kanda
Jun 12, 2016·Advances in Therapy·Dennis HernandezFiona McPhee
Jun 25, 2016·Viruses·Ahmed Atef MesalamPhilip Meuleman
Jun 18, 2015·PloS One·Satoshi YoshimiKazuaki Chayama
Aug 5, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tomomi OkuboKatsuhiko Iwakiri
Nov 27, 2015·The Lancet Infectious Diseases·Regis A Vilchez
Feb 9, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Masahiro ShinodaYuko Kitagawa
Oct 25, 2016·PloS One·Jun ItakuraNamiki Izumi
Oct 27, 2016·Journal of Medicinal Chemistry·Xing-Yue JiZhuo-Rong Li
May 12, 2016·PloS One·Jesper WaldenströmMartin Lagging
Jan 31, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Christophe HézodeStefan Zeuzem
Apr 26, 2017·International Journal of Molecular Sciences·Tatsuo KandaOsamu Yokosuka
Nov 13, 2015·Viruses·Celia PeralesEsteban Domingo
Jan 2, 2018·World Journal of Gastroenterology : WJG·Tatsuo KandaMitsuhiko Moriyama
Feb 6, 2019·BMC Nephrology·Michael DuerrFabian Halleck
Apr 28, 2018·Viruses·Ruben BrandãoPerpétua Gomes
Jan 10, 2017·The American Journal of Case Reports·Hiroshi NakagomiMasao Omata
Nov 11, 2019·BMC Gastroenterology·Hiroshi OhyamaMasao Omata
Nov 5, 2019·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Zhenqiu LiuXingdong Chen