HCV RNA measurement in samples with diverse genotypes using versions 1 and 2 of the Roche COBAS® AmpliPrep/COBAS® TaqMan® HCV test

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Michael T PyneDavid R Hillyard

Abstract

Accurate HCV RNA measurement is required for monitoring treatment. Underquantification has been reported with some genotypes, particularly genotype 4, using version 1 of the Roche COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HCV Test. Compare the viral loads of clinical specimens representing diverse genotypes from across the United States using versions 1 (V1) and 2 (V2) of the Roche COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HCV Test. Assess the frequency of nt145 and nt165 variants in the 5' UTR associated with detection failures and underquantification in a large clinical sample database. Three hundred archived clinical samples were measured using V1 and V2. Bland-Altman analysis was performed on log-transformed results and compared by genotype. The frequencies of nt145 and nt165 variants from 15,817 sequences were calculated. On average, V2 results were 0.16 logIU/mL lower than V1 results. The average genotype 4 sample was 0.08 logIU/mL higher in V1 than V2. The largest individual sample differences were -2.10 (genotype 2b) and 1.57 (genotype 2a) logIU/mL. For genotype 4 samples, the greatest underquantification by V1 was 1.46 logIU/mL. There were 13 (0.082%) variants at nt145 and 24 variants at nt165 (0.152%), including one sequence ...Continue Reading

References

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May 26, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stéphane ChevaliezJean-Michel Pawlotsky
Aug 12, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christoph SarrazinAlexandra Valsamakis
Mar 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stéphane ChevaliezJean-Michel Pawlotsky
Oct 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jeffrey J GermerJoseph D C Yao
Mar 17, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philippe HalfonPatrick Marcellin
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sepideh AkhavanVincent Thibault
Jun 29, 2013·Diagnostic Microbiology and Infectious Disease·Michael T Pyne, David R Hillyard
Mar 25, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Melanie A MalloryDavid R Hillyard
Jun 5, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kristian Schønning

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Citations

Dec 19, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Virginia M PierceEric S Rosenberg
Aug 17, 2018·Journal of Viral Hepatitis·Akash SinghVirendra Singh
Oct 8, 2020·Liver International : Official Journal of the International Association for the Study of the Liver·Sunil TanejaHarbir S Kohli

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