HDAC3 and HDAC8 are required for cilia assembly and elongation

Biology Open
Seon-Ah ParkKunsoo Rhee

Abstract

Cilia are extended from mother centrioles in quiescent G0/G1 cells and retracted in dividing cells. Diverse post-translational modifications play roles in the assembly and disassembly of the cilium. Here, we examined class I histone deacetylases (HDACs) as positive regulators of cilia assembly in serum-deprived RPE1 and HK2 cells. We observed that the number of cells with cilia was significantly reduced in HDAC3- and HDAC8-depleted cells. The ciliary length also decreased in HDAC3- and HDAC8-depleted cells compared to that in control cells. A knockdown-rescue experiment showed that wild-type HDAC3 and HDAC8 rescued the cilia assembly and ciliary length in HDAC3- and HDAC8-depleted cells, respectively; however, deacetylase-dead HDAC3 and HDAC8 mutants did not. This suggests that deacetylase activity is critical for both HDAC3 and HDAC8 function in cilia assembly and ciliary length control. This is the first study to report that HDACs are required for the assembly and elongation of the primary cilia.

References

Jan 10, 2002·The Journal of Biological Chemistry·Wen-Ming YangEdward Seto
Oct 2, 2002·The Journal of Biological Chemistry·Hirotaka SakaiFuyuki Ishikawa
Dec 13, 2006·Molecular and Cellular Biology·Serge GrégoireXiang-Jiao Yang
Jul 3, 2007·Cell·Elena N PugachevaErica A Golemis
Feb 23, 2008·Nature Reviews. Molecular Cell Biology·Xiang-Jiao Yang, Edward Seto
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ying CaoZhaoxia Sun
Apr 17, 2010·Nature Reviews. Genetics·Sarah C Goetz, Kathryn V Anderson
Sep 11, 2010·Nature·Jyothi S AkellaJacek Gaertig
Sep 30, 2010·Development·Susana S LopesLeonor Saúde
Nov 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Toshinobu ShidaMaxence V Nachury
Jan 11, 2012·Nature·Peter J WatsonJohn W R Schwabe
May 25, 2012·Molecular Biology of the Cell·Olga V PlotnikovaErica A Golemis
Oct 2, 2012·Cell Cycle·Hongbo LingKenji Fukasawa
Feb 13, 2013·The Journal of Cell Biology·Stefanie KuhnsGislene Pereira
Dec 4, 2013·Nature Cell Biology·Ching-Hwa Sung, Michel R Leroux
Jun 6, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Liwei Huang, Joshua H Lipschutz
Jun 13, 2014·American Journal of Physiology. Cell Physiology·Jia LiDale D Tang
May 12, 2015·Cell Reports·Janos TözserYoichi Kato
Aug 1, 2015·Cellular and Molecular Life Sciences : CMLS·Lin Li, Xiang-Jiao Yang
Aug 21, 2015·Nature Communications·Sehyun KimBrian David Dynlacht
Jan 26, 2016·Biochemistry·Sister M Lucy GanttCarol A Fierke
Jun 29, 2016·Nature Cell Biology·Irma Sánchez, Brian David Dynlacht
Dec 29, 2016·EMBO Reports·Tetsuo KobayashiHiroshi Itoh
Feb 23, 2017·Oncotarget·Kemei ZhangBo Xiong

❮ Previous
Next ❯

Citations

Aug 14, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Li Eon Kuek, Robert J Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
transfection

Software Mentioned

ImagePro
ImageJ
GraphPad Prism
NeuronJ
SigmaPlot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.