HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model

Neuropsychiatric Disease and Treatment
Yinian ZhangYa-Wen Pan

Abstract

Despite the availability of effective antiepileptic drugs, epileptic patients still suffer from intractable seizures and adverse events. Better control of both seizures and fewer side effects is needed in order to enhance the patient's quality of life. We performed the present study with an attempt to explore the effect that HDAC4 gene silencing would have on epilepsy simulated by model rats. Furthermore, the study made additional analysis on the relativity of the HDAC4 gene in regard to its relationship with the gamma-aminobutyric acid (GABA) signaling pathway. Tremor rats were prepared in order to establish the epilepsy model. The rats would go on to be treated with si-HDAC4 in order to identify roles of the HDAC4 in levels of GABAARα1, GABAARα4, GAD65, GAT-1, and GAT-3. Finally, both electroencephalogram behavior and cognitive function of the rats following the treatment of si-HDAC4 were observed. Levels of the GABAARα1 and GABAARα4 showed an evident increase, while GAD65, GAT-1, and GAT-3 displayed a decline in the epilepsy rats treated with the aforementioned si-HDAC4 when compared with the epilepsy rats. After injection of si-HDAC4, the epilepsy rats presented with a reduction in seizure degree, latency and duration of se...Continue Reading

Citations

Dec 5, 2020·Expert Opinion on Therapeutic Targets·Sonali KumarAparna Banerjee Dixit
Sep 21, 2021·Epilepsia Open·Daniel Fonseca-BarriendosLuisa Rocha

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Methods Mentioned

BETA
acetylation
nuclear magnetic resonance
reverse transcription PCR
transfection

Software Mentioned

ImageJ
Primer5
SPSS18
RNAseq

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