PMID: 19942787Nov 28, 2009Paper

HDL2 can inhibit further oxidative modification of partially oxidized LDL

Journal of Atherosclerosis and Thrombosis
Nagahiko SakumaSyuichi Kitada

Abstract

To investigate whether HDL(2) can inhibit further oxidative modification of partially oxidized LDL (ox-LDL) by interrupting the chain oxidation reaction after lipid hydroperoxides (LOOH) formation. Following incubation of LDL 400 microg protein/mL phosphate-buffered saline with Cu(2+) for 1.75 h (defined as 0 min), incubation was continued after adding HDL(2) 200 microg protein/mL or HDL(2) 800 microg protein/mL to give both ox-LDL+HDL(2) 200 microg protein/mL or ox-LDL+HDL(2) 800 microg protein/mL. As a control, ox-LDL 200 microg protein/mL and native LDL were prepared. Each sample was subjected to agarose gel electrophoresis and the LOOH in each sample was measured. When the electrophoretic mobility of native LDL was designated 1, the relative electrophoretic mobility (REM) of ox-LDL increased significantly over time. The REMs of ox-LDL+HDL(2) 800 microg protein/mL from 10 min to 9 h were significantly lower than the REM of ox-LDL at the respective times (p<0.01). LOOH of ox-LDL+HDL(2) 800 microg protein/mL at 1, 3, 6 and 9 h was significantly higher than LOOH in ox-LDL at the respective times (p<0.01). The results of ox-LDL+HDL(2) 200 microg protein/mL were almost the same but to a lesser extent than the results of ox-LDL+HD...Continue Reading

References

Feb 1, 1991·Clinical Cardiology·C J SchwartzR M Nerem
Mar 1, 1990·Chemical Research in Toxicology·H EsterbauerG Jürgens
Oct 1, 1990·Biochemical Pharmacology·R Chander, N K Kapoor
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Nov 9, 1989·The New England Journal of Medicine·D J Gordon, B M Rifkind
Mar 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·U P SteinbrecherD Steinberg
Nov 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·P W WilsonW P Castelli
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M T QuinnD Steinberg
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M T QuinnD Steinberg
Jul 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·D W MorelG M Chisolm
Mar 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T HenriksenD Steinberg
Jun 1, 1995·Atherosclerosis·M I Mackness, P N Durrington
Apr 1, 1993·Atherosclerosis·A N KlimovN P Teryukova
Jun 29, 2000·Journal of Atherosclerosis and Thrombosis·W B Kannel
Jul 6, 2000·The Journal of Laboratory and Clinical Medicine·N ChenJ L Holtzman
Apr 17, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·P N DurringtonM I Mackness
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON
Sep 8, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Mohamad NavabAlan M Fogelman
Apr 4, 2008·Journal of Atherosclerosis and Thrombosis·Dmitri SviridovPaul Nestel

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.