PMID: 9187302May 17, 1997Paper

HDL3-signalling in HepG2 cells involves glycosyl-phosphatidylinositol-anchored proteins

Biochimica Et Biophysica Acta
F Nazih-SandersonC Delbart

Abstract

In [3H]phosphatidylcholine (PC) prelabelled HepG2 cells, HDL3 stimulates a biphasic increase in 1.2-diacylglycerol (DAG). The early phase is mediated in part by a phospholipase C which is inhibited by 10 microM D 609, RHC-80267 or U-73122 and less by 100 microM propranolol. A phospholipase D is more likely involved in the late phase, as the DAG peak lags behind phosphatidic acid rise and is blocked by 100 microM propranolol. Cellular preincubation with 200 microg/ml antibodies against the inositolphosphoglycan (IPG) moiety of the GPI-anchor (Ab(IPG)), or depletion in GPI-anchored proteins by cellular pretreatment with 0.5 U/ml PI-PLC, 1 mM insulin and 2 HU/ml streptolysin-O, or depletion in membrane cholesterol content by filipin (5 microg/ml), digitonin (5 microg/ml) and cholesterol oxidase (0.5 U/ml) decreases the HDL3-signal, suggesting the involvement of a lipolytic cleavage of GPI-anchored proteins. Inhibition of proteases by 1 mM leupeptin/PMSF improves the response time to HDL3, with a DAG peak at 2-3 min. In the presence of protease-inhibitors, HDL3 releases in the culture medium several proteins with a residual IPG that binds Ab(IPG) after SDS-PAGE analysis and immunoblotting. HDL3-signalling pathways comprise tyrosine...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1976·The Biochemical Journal·P M Starkey, A J Barrett
Aug 30, 1991·Biochemical and Biophysical Research Communications·O HolianB Attar
Sep 15, 1990·Thrombosis Research·H NazihC Delbart
Dec 31, 1990·Biochemical and Biophysical Research Communications·N TheretG Ailhaud
Jan 1, 1990·Archives of Biochemistry and Biophysics·E R Lazarowski, E G Lapetina
Dec 6, 1989·Biochimica Et Biophysica Acta·M G Low
Nov 7, 1995·Biochemistry·P E Fielding, C J Fielding
Sep 8, 1995·Science·R G Parton, K Simons
Jun 1, 1995·Atherosclerosis·M I Mackness, P N Durrington
Apr 14, 1995·Science·P J Casey
Jan 1, 1995·Molecular Membrane Biology·M P LisantiM Sargiacomo
Nov 1, 1994·Trends in Biochemical Sciences·C MacKintosh, R W MacKintosh
Feb 6, 1995·Biochemical and Biophysical Research Communications·J R NoferG Assmann
Dec 1, 1994·The Journal of Cell Biology·E J SmartR G Anderson
Aug 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H NazihC Delbart
Aug 11, 1994·Biochimica Et Biophysica Acta·C BenistantR Rubin
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Anderson
Jan 11, 1994·Biochimica Et Biophysica Acta·M N PietersT J Van Berkel
Sep 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·V N BochkovT J Resink
Oct 1, 1993·Metabolism: Clinical and Experimental·V NivetD Durand
Jan 1, 1993·Advances in Pharmacology·G Romero, J Larner
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Feb 7, 2015·Essays in Biochemistry·Parham Ashrafzadeh, Ingela Parmryd
Feb 12, 1998·Biochemical and Biophysical Research Communications·G LebuffeP Duriez
Aug 21, 1997·Biochimica Et Biophysica Acta·F Nazih-SandersonC Delbart

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