Hdm2 and nitric oxide radicals contribute to the p53-dependent radioadaptive response

International Journal of Radiation Oncology, Biology, Physics
Akihisa TakahashiTakeo Ohnishi

Abstract

The aim of this work was to characterize the radioadaptive response at the molecular level. We used wild-type (wt) p53 and mutated (m) p53-containing cells derived from the human lung cancer H1299 cell line, which is p53-null. Cellular radiation sensitivities were determined with a colony-forming assay. The accumulations of p53, the human homolog of endogenous murine double minute 2 (Hdm2), and inducible nitric oxide synthase were analyzed with Western blotting. Quantification of chromosomal aberrations was estimated by scoring dicentrics per cell. In wtp53 cells, it was demonstrated that the lack of p53 accumulation was coupled with the activation of Hdm2 after low-dose irradiation (0.02 Gy). Although NO radicals were only minimally induced in wtp53 cells irradiated with a challenging irradiation (6 Gy) alone, NO radicals were seen to increase about two- to fourfold after challenging irradiation subsequent to a priming irradiation (0.02 Gy). Under similar irradiation conditions with a priming and challenging irradiation in wtp53 cells, induction of radioresistance and a depression of chromosomal aberrations were observed only in the absence of 5, 5'-(2, 5-Furanidiyl)bis-2-thiophenemethanol (RITA) or Nutlin-3 (p53-Hdm2 interact...Continue Reading

References

Jun 1, 1967·Health Physics·S E Kim, W S Moos
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·T P KastenG A Nickols
Jul 1, 1993·Genes & Development·X WuA J Levine
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·K ForresterC C Harris
Nov 4, 1996·Mutation Research·H E Kleczkowska, F R Althaus
May 15, 1997·Nature·M H KubbutatK H Vousden
Feb 7, 2001·International Journal of Radiation Biology·A BravardO Rigaud
Feb 11, 2000·Radiation Research·S H ParkY S Lee
May 15, 2001·The Journal of Biological Chemistry·R A JohnsonK W Scotto
Sep 29, 2001·International Journal of Radiation Biology·A TakahashiT Ohnishi
May 15, 2002·Radiation Research·Chang-Mo KangYun-Sil Lee
Jul 11, 2002·Mutation Research·Masao S SasakiTaisei Nomura
Aug 27, 2002·International Journal of Radiation Biology·A TakahashiT Ohnishi
Mar 18, 2003·Molecular and Cellular Biology·Guozheng GuoJian Jian Li
Jan 6, 2004·Science·Lyubomir T VassilevEmily A Liu
Sep 24, 2004·International Journal of Radiation Oncology, Biology, Physics·Akihisa TakahashiTakeo Ohnishi
Apr 29, 2005·Uchū Seibutsu Kagaku·Hideki MatsumotoTakeo Ohnishi
Sep 29, 2005·Radiation Research·Patricia CramersJos C S Kleinjans
Sep 13, 2006·International Journal of Radiation Biology·Bhavani ShankarKrishna Sainis

❮ Previous
Next ❯

Citations

Jan 13, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Edward J Calabrese
Aug 24, 2010·International Journal of Radiation Oncology, Biology, Physics·Akihisa TakahashiTakeo Ohnishi
Oct 14, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Shuang YeChunlin Shao
May 23, 2013·International Journal of Radiation Biology·Shuang YeChunlin Shao
Jun 19, 2012·International Journal of Radiation Biology·Irena Szumiel
Jan 8, 2013·Radiation Research·Masanori TomitaHideki Matsumoto
Jan 25, 2019·International Journal of Radiation Biology·Kavitha PremkumarBhavani S Shankar
Jun 16, 2009·Journal of Radiation Research·Akihisa Takahashi, Takeo Ohnishi
Sep 17, 2011·Journal of Radiation Research·Guillaume VaresMitsuru Nenoi
May 16, 2009·Journal of Radiation Research·Hideki MatsumotoMasanori Hatashita
Feb 5, 2011·Journal of Radiation Research·Guillaume VaresMitsuru Nenoi
Jul 16, 2011·Journal of Radiation Research·Xiaoming SuTakeo Ohnishi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Antianginal Drugs: Mechanisms of Action

Antianginal drugs, including nitrates, beta-blockers, and calcium channel blockers, are used in the treatment of angina pectoris. Here is the latest research on their use and their mechanism of action.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis