HDP2: a ribosomal DNA (NTS-ETS) sequence as a target for species-specific molecular diagnosis of intestinal taeniasis in humans

Parasites & Vectors
María D FloresTeresa Gárate

Abstract

Taenia solium, T. asiatica and T. saginata tapeworms cause human taeniasis and are the origin of porcine and bovine cysticercosis. Furthermore, T. solium eggs can cause human cysticercosis, with neurocysticercosis being the most serious form of the disease. These helminth infections are neglected tropical diseases and are endemic in several countries in the Americas, Asia and Africa. As a result of globalization, migration in particular, the infections have been extending to non-endemic territories. Species-specific diagnosis of taeniasis is subject to drawbacks that could be resolved using molecular approaches. In the present study, conventional and real-time amplification protocols (cPCR and qPCR) based on the T. saginata HDP2 sequence were applied in the differential diagnosis of taeniasis (T. saginata, T. solium) in both fecal samples and proglottids expelled by patients. The HDP2 homolog in T. solium was cloned and characterized. Semi-nested cPCR and qPCR (Sn-HDP2 cPCR and Sn-HDP2 qPCR) amplified T. saginata and T. solium DNA, with an analytical sensitivity of 40 and 400 fg, respectively, and identically in both protocols. Eighteen taeniasis patients were diagnosed directly with T. saginata or T. solium, either from proglo...Continue Reading

References

Jun 1, 1990·Parasitology·L J HarrisonR M Parkhouse
Mar 11, 1986·Nucleic Acids Research·R E EllisA R Coulson
Apr 25, 1997·Journal of Molecular Biology·C Burge, S Karlin
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 3, 2000·Journal of Clinical Microbiology·L M GonzálezT Garate
May 30, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E C GoulartA L Rodrigues
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Luis Miguel GonzálezTeresa Garate
Mar 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E MonteroT Gárate
Aug 23, 2003·Lancet·Héctor H GarcíaUNKNOWN Cysticercosis Working Group in Peru
Aug 23, 2003·Experimental Parasitology·Cáris Maroni NunesJosé Fernando Garcia
Feb 10, 2004·Journal of Clinical Microbiology·Hiroshi YamasakiAkira Ito
Jul 13, 2004·Diagnostic Microbiology and Infectious Disease·Luis Miguel GonzálezTeresa Gárate
Sep 1, 2007·Current Opinion in Infectious Diseases·Philip Craig, Akira Ito
May 31, 2008·Annals of Tropical Medicine and Parasitology·M HernándezE Sciutto
Nov 14, 2008·Journal of Clinical Microbiology·Agathe NkouawaAkira Ito
Aug 8, 2013·PloS One·Lorenzo ZammarchiUNKNOWN COHEMI Project Study Group
Oct 28, 2015·Acta Tropica·Francisco Rogerlândio Martins-MeloJorg Heukelbach
Dec 26, 2015·Experimental Parasitology·Jeroen H RoelfsemaLaetitia M Kortbeek
Jun 16, 2016·The New England Journal of Medicine·Hector H GarciaUNKNOWN Cysticercosis Working Group in Peru
Jul 3, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M A Gómez-MoralesA S Winkler
Jul 25, 2017·Parasites & Vectors·Minerva Laranjo-GonzálezVeronique Dermauw

❮ Previous
Next ❯

Citations

Jan 11, 2019·Parasitology Research·Ana Vargas-CallaUNKNOWN Cysticercosis Working Group in Peru

❮ Previous
Next ❯

Datasets Mentioned

BETA
KY750551

Methods Mentioned

BETA
ELISA
PCRs
PCR
optical microscopy
electrophoresis
amplicon sequencing

Software Mentioned

BLAST

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Related Papers

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Yi-Mei YangKang-Huai He
Diagnostic Microbiology and Infectious Disease
Luis M GonzalezTeresa Gárate
Advances in Parasitology
Z Pawlowski, M G Schultz
© 2022 Meta ULC. All rights reserved