PMID: 346614Apr 1, 1978

Head-space gas liquid chromatography for rapid detection of Escherichia coli and Proteus mirabilis in urine

Journal of Clinical Pathology
P J Coloe

Abstract

Measuring ethanol formed from arabinose by Escherichia coli and dimethyl disulphide formed from methionine by Proteus mirabilis is a rapid way of detecting these organisms in the urine. Ethanol and dimethyl disulphide are detected by head-space gas-liquid chromatography. Since sedimented organisms and not whole urine are used the method is not subject to interference from compounds within the urine. When it was tested on 122 samples of urine, 94 from patients with suspected urinary infection and 28 supposedly uninfected control specimens, the results correlated well with viable counts by a conventional bacteriological method. As well as being rapid (results available in four hours) the method is reliable, easy to use, and could be automated. It now requires extensive testing in a hospital laboratory.

References

Nov 29, 1975·Lancet·R V Mummery
Apr 1, 1978·Journal of Clinical Pathology·P J Coloe
Nov 1, 1938·The Journal of Hygiene·A A MilesJ O Irwin

Citations

Jul 5, 2013·Clinical Microbiology Reviews·Shneh SethiTrinad Chakraborty
Jan 1, 1991·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·J StrassburgerR Seidel
May 16, 2013·PLoS Pathogens·Lieuwe D J BosMarcus J Schultz
Sep 28, 2017·Journal of Breath Research·Christiaan A ReesJane E Hill
Jun 1, 1981·Journal of Religion and Health·H Vande Kemp, G P Schreck
Jun 2, 2012·Cognition & Emotion·Meagan M GraydonDennis R Proffitt
Jul 29, 2020·Biosensors·Valentin-Mihai DospinescuJames A Covington

Related Concepts

Ethanol
Bacteriological Techniques
Bacteriuria
Chromatography, Gas-Liquid
Disulfides
Alkalescens-Dispar Group
Klebsiella rhinoscleromatis
Proteus mirabilis
Proteus vulgaris

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