Head-to-head comparison of in-house produced CD19 CAR-T cell in ALL and NHL patients

Journal for Immunotherapy of Cancer
Orit ItzhakiMichal J Besser

Abstract

CD19 chimeric antigen receptor T (CAR-T) cells demonstrate remarkable remission rates in pediatric and adult patients with refractory or relapsed (r/r) acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). In 2016, we initiated a clinical trial with in-house produced CD19 CAR-T cells with a CD28 co-stimulatory domain. We analyzed, for the first time, differences in production features and phenotype between ALL and NHL patients. Non-cryopreserved CAR-T cells were produced from patients' peripheral blood mononuclear cells within 9 to 10 days. 93 patients with r/r ALL and NHL were enrolled under the same study. CAR-T cells of ALL and NHL patients were produced simultaneously, allowing the head-to-head comparison. All patients were heavily pretreated. Three patients dropped out from the study due to clinical deterioration (n=2) or production failure (n=1). Cells of ALL patients (n=37) expanded significantly better and contained more CAR-T cells than of NHL patients (n=53). Young age had a positive impact on the proliferation capacity. The infusion products from ALL patients contained significantly more naïve CAR-T cells and a significantly higher expression of the chemokine receptor CXCR3. PD-1, LAG-3, TIM-3, and CD2...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G GrossZ Eshhar
Dec 31, 1987·Biochemical and Biophysical Research Communications·Y KuwanaY Kurosawa
Oct 13, 2006·Blood·Adele K FieldingUNKNOWN Eastern Cooperative Oncology Group
Sep 24, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark E DudleySteven A Rosenberg
Jun 30, 2009·Journal of Immunotherapy·James N KochenderferSteven A Rosenberg
Apr 22, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michal J BesserJacob Schachter
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marleen M van LoenenMirjam H M Heemskerk
Oct 30, 2010·CA: a Cancer Journal for Clinicians·Christopher R FlowersJulie M Vose
Apr 19, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Steven A RosenbergMark E Dudley
Dec 23, 2011·Genes & Development·Stephen L ShiaoLisa M Coussens
May 8, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark E DudleySteven A Rosenberg
May 22, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michal J BesserJacob Schachter
Nov 20, 2013·British Journal of Haematology·Dai ChiharaKeitaro Matsuo
Aug 27, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·James N KochenderferSteven A Rosenberg
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Oct 16, 2014·The New England Journal of Medicine·Shannon L MaudeStephan A Grupp
Jan 1, 2014·Molecular Therapy. Methods & Clinical Development·Pradip BajgainJuan F Vera
Aug 26, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ching-Hon PuiCharles G Mullighan
Dec 15, 2015·Science Translational Medicine·Giacomo OliveiraChiara Bonini
May 25, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stephanie L GoffSteven A Rosenberg
Mar 28, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Robert SacksteinSteven R Barthel
Dec 12, 2017·The New England Journal of Medicine·Stephen J SchusterCarl H June
Feb 1, 2018·The New England Journal of Medicine·Shannon L MaudeStephan A Grupp
May 26, 2018·Bone Marrow Transplantation·Abraham AvigdorArnon Nagler
Jun 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie-Andrée ForgetChantale Bernatchez
Dec 7, 2018·The New England Journal of Medicine·Stephen J SchusterUNKNOWN JULIET Investigators
Mar 13, 2019·The Journal of Clinical Investigation·Olivia C FinneyMichael C Jensen

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Citations

Jul 28, 2020·Frontiers in Immunology·Meir RozenbaumMichal J Besser
Jan 26, 2021·Frontiers in Immunology·Hadas Weinstein-MaromMichal J Besser
Mar 20, 2021·Bulletin of Mathematical Biology·Katherine Owens, Ivana Bozic
May 16, 2021·Journal for Immunotherapy of Cancer·Abraham NissaniMichal J Besser
Jul 30, 2021·Stem Cell Research & Therapy·Ehsan RazeghianFarhad Motavalli Khiavi
Sep 14, 2021·The Cochrane Database of Systematic Reviews·Moritz ErnstMarius Goldkuhle

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Methods Mentioned

BETA
leukapheresis
biopsies
ELISA
FACS

Software Mentioned

FlowJo

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