Healthy but not RSV-infected lung epithelial cells profoundly inhibit T cell activation.

Thorax
H WangJ Schwarze

Abstract

Respiratory viruses, including respiratory syncytial virus (RSV), can cause asthma exacerbations and bronchiolitis. Both conditions are associated with enhanced cognate immune responses and inflammation and reduced immune regulation. Lung epithelial cells (LECs) can contribute to antiviral and allergic immune responses while gut epithelial cells can inhibit effector T cell responses. A study was performed to determine whether healthy LECs regulate antigen-specific T cell responses and if this regulation is lost during RSV infection. LA4 cells, a murine LEC line, infected with RSV or primary murine LECs were co-cultured with ovalbumin-specific T cell receptor transgenic CD4+ T cells from DO11.10 mice and ovalbumin-pulsed bone marrow-derived dendritic cells (DC) to assess T cell proliferation by flow cytometry and cytokine production. The presence of LECs abrogated DC-induced T cell proliferation and significantly reduced T cell cytokine release. These effects of LECs were predominantly contact-dependent, primarily affected T cells directly and were partly mediated by transforming growth factor beta. Soluble factors and DC-mediated effects also contributed to T cell inhibition. RSV infection of LECs reduced their inhibitory capac...Continue Reading

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Citations

Jul 7, 2010·Immunology and Cell Biology·Jianou QiaoXianqiao Jin
Dec 15, 2010·The Journal of Infectious Diseases·Aurica G TelcianSebastian L Johnston
Jan 15, 2011·Clinical Microbiology Reviews·Marjolaine VareilleNicolas Regamey
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Apr 4, 2019·Frontiers in Pediatrics·Andrew Bush
Aug 9, 2021·Molecular Aspects of Medicine·Zsolt I KomlósiCezmi A Akdis
Aug 19, 2021·Expert Reviews in Molecular Medicine·Michael W MatherJason Powell

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
transgenic
flow cytometry
ELISA

Software Mentioned

GraphPad
GraphPad Prism
Revelation
GraphPad Instat
WinList
CellQuest

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