Heart failure decreases passive tension generation of rat diaphragm fibers

International Journal of Cardiology
Hieronymus W H van HeesLeo M A Heunks

Abstract

Diaphragm dysfunction is well-known to limit quality of life and prognosis of patients with heart failure (HF), but its underlying mechanisms are not well understood. In an animal model for HF we recently showed that impaired diaphragm contractility arises at the single fiber level and is associated with sarcomeric injuries. For optimal muscle function and sarcomeric stability passive elastic structures, like titin, are indispensable. The current study aimed to investigate if impaired passive elasticity contributes to diaphragm dysfunction in rats with heart failure. Skinned muscle fibers were isolated from the diaphragm and soleus of rats with chronic HF, induced by left coronary artery ligation and of sham-operated rats. Passive tension-length relationships were determined by applying segmental extension tests. Immunofluorescence was performed on muscle cryosections using antibodies (T12) against a titin epitope near the Z-line. Titin content was determined by SDS-agarose-gel electrophoresis. Titin's mobility on gel was studied to detect changes in titin size. Passive tension generation upon stretch was significantly reduced (>35%) in HF diaphragm fibers compared to sham. Immunostaining intensities against titin were reduced ...Continue Reading

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Citations

Dec 7, 2010·American Journal of Respiratory and Critical Care Medicine·Frances S de ManCoen A C Ottenheijm
Sep 5, 2013·Thyroid : Official Journal of the American Thyroid Association·Antonio C BiancoUNKNOWN American Thyroid Association Task Force on Approaches and Strategies to Investigate Thyroid Hormone Economy and Action
Feb 14, 2012·Intensive Care Medicine·Hieronymus W H van HeesLeo M A Heunks
Sep 28, 2016·PeerJ·Fausto Antonio PanizzoloJonas Rubenson
Jul 13, 2013·Journal of Applied Physiology·Debby ThomasGhislaine Gayan-Ramirez
Aug 9, 2017·Canadian Journal of Physiology and Pharmacology·Rodrigo B JaenischPedro Dal Lago
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Jan 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T Scott BowenVolker Adams
May 3, 2019·American Journal of Physiology. Cell Physiology·Robbert J van der PijlCoen A C Ottenheijm

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