Heart failure: Pilot transcriptomic analysis of cardiac tissue by RNA-sequencing

Cardiology Journal
Concetta SchianoClaudio Napoli

Abstract

Despite left ventricular (LV) dysfunction contributing to mortality in chronic heart failure (HF), the molecular mechanisms of LV failure continues to remain poorly understood and myocardial biomarkers have yet to be identified. The aim of this pilot study was to investigate specific transcriptome changes occurring in cardiac tissues of patients with HF compared to healthy condition patients to improve diagnosis and possible treatment of affected subjects. Unlike other studies, only dilated cardiomyopathy (DCM) (n = 2) and restrictive cardiomyopathy (RCM) (n = 2) patients who did not report family history of the disease were selected with the aim of obtaining a homogeneous population for the study. The transcriptome of all patients were studied by RNA-sequencing (RNA-Seq) and the read counts were adequately filtered and normalized using a recently developed user-friendly tool for RNA-Seq data analysis, based on a new graphical user interface (RNA-SeqGUI). By using this approach in a pairwise comparison with healthy donors, we were able to identify DCM- and RCM-specific expression signatures for protein-coding genes as well as for long noncoding RNAs (lncRNAs). Differential expression of 5 genes encoding different members of the...Continue Reading

Citations

Oct 16, 2019·Journal of Clinical Pathology·Giuditta BenincasaClaudio Napoli
Oct 14, 2017·Annals of Medicine·Samira KalayiniaNejat Mahdieh
Oct 26, 2018·Circulation Research·Weiqi ZhangGuang-Hui Liu
Jun 12, 2019·Heart Failure Reviews·Michael SarheneFan Guanwei
Sep 2, 2018·Journal of Cardiovascular Translational Research·Yongqin LiYihua Bei
Feb 28, 2020·Pharmacology & Therapeutics·Concetta SchianoClaudio Napoli
Mar 4, 2021·Forensic Science International. Genetics·Cordula HaasJack Ballantyne
Apr 1, 2021·Journal of the American Heart Association·Ricardo O Ramirez FloresJulio Saez-Rodriguez
Mar 26, 2018·AIMS Genetics·Carlos García-PadillaDiego Franco
Jun 3, 2021·International Journal of Environmental Research and Public Health·Sek Ying ChairWai Tong Chien
Aug 28, 2021·International Journal of Molecular Sciences·Christina Pagiatakis, Vittoria Di Mauro

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