Heart infarct in NOD-SCID mice: therapeutic vasculogenesis by transplantation of human CD34+ cells and low dose CD34+KDR+ cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Rosanna BottaC Peschle

Abstract

Hematopoietic (Hem) and endothelial (End) lineages derive from a common progenitor cell, the hemangioblast: specifically, the human cord blood (CB) CD34+KDR+ cell fraction comprises primitive Hem and End cells, as well as hemangioblasts. In humans, the potential therapeutic role of Hem and End progenitors in ischemic heart disease is subject to intense investigation. Particularly, the contribution of these cells to angiogenesis and cardiomyogenesis in myocardial ischemia is not well established. In our studies, we induced myocardial infarct (MI) in the immunocompromised NOD-SCID mouse model, and monitored the effects of myocardial transplantation of human CB CD34+ cells on cardiac function. Specifically, we compared the therapeutic effect of unseparated CD34+ cells vs. PBS and mononuclear cells (MNCs); moreover, we compared the action of the CD34+KDR+ cell subfraction vs. the CD34+KDR- subset. CD34+ cells significantly improve cardiac function after MI, as compared with PBS/MNCs. Similar beneficial actions were obtained using a 2-log lower number of CD34+KDR+ cells, while the same number of CD34+KDR- cells did not have any effects. The beneficial effect of CD34+KDR+ cells may mostly be ascribed to their notable resistance to ap...Continue Reading

References

May 4, 1999·The Journal of Clinical Investigation·J M Isner, T Asahara
Sep 8, 1999·Science·B L ZieglerC Peschle
Apr 5, 2001·Nature·D OrlicP Anversa
Jun 8, 2001·The Journal of Clinical Investigation·K A JacksonM A Goodell
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·G CondorelliC M Croce
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·D OrlicP Anversa
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·G CondorelliG Cossu
Jan 5, 2002·The New England Journal of Medicine·Federico QuainiPiero Anversa
Apr 6, 2002·Circulation Research·Michael A LaflammeCharles E Murry
Oct 18, 2002·Blood·Elvira PelosiCesare Peschle
Oct 14, 2003·Cardiovascular Research·Takeshi TsudaMon-Li Chu
Oct 14, 2003·Nature·Manuel Alvarez-DoladoArturo Alvarez-Buylla

❮ Previous
Next ❯

Citations

Apr 30, 2010·Cardiovascular Research·Gian Paolo Fadini, Angelo Avogaro
Sep 13, 2012·Journal of Transplantation·Emily Sims, Carmella Evans-Molina
Jan 31, 2007·Journal of Translational Medicine·Neil H RiordanThomas E Ichim
Jan 5, 2011·Frontiers in Bioscience (Elite Edition)·Dale S AdlerVincent J Pompili
Jun 18, 2009·Seminars in Immunopathology·Ping ZhouJan Nolta
Nov 3, 2010·Pharmacology & Therapeutics·Maurizio PesceMaurizio C Capogrossi
Sep 15, 2010·Best Practice & Research. Clinical Haematology·Hadar Arien-ZakayArnon Nagler
Apr 7, 2009·Trends in Cardiovascular Medicine·Guido KrenningMartin C Harmsen
Mar 24, 2009·Trends in Molecular Medicine·Guido KrenningMartin C Harmsen
Oct 1, 2008·International Journal of Cardiology·Giulio PompilioPaolo Biglioli
Oct 24, 2007·Microvascular Research·Marie-Ange Renault, Douglas W Losordo
Sep 25, 2007·Experimental Cell Research·Gloria InverniciEugenio A Parati
Mar 21, 2007·Journal of Molecular and Cellular Cardiology·Jonathan L GoldbergVincent J Pompili
Jan 30, 2007·Trends in Molecular Medicine·Antonia GermaniMaurizio C Capogrossi
Aug 12, 2005·Annals of the New York Academy of Sciences·Cesare Peschle, Gianluigi Condorelli
Jun 21, 2012·British Journal of Pharmacology·T JadczykP Madeddu
Oct 27, 2004·Experimental Hematology·Christopher R Cogle, Edward W Scott
Jul 22, 2014·Stem Cell Research·Kathy O LuiKenneth R Chien
Mar 14, 2007·Cytotherapy·J L Goldberg, M J Laughlin
May 11, 2007·Journal of Molecular and Cellular Cardiology·B W A van der StrateM J A van Luyn
Apr 20, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V Pinho-RibeiroA C Campos de Carvalho
Dec 22, 2017·Journal of Cellular Biochemistry·Ayman El-Sayed ShafeiAmal S El-Shal
Jan 1, 2012·Cells·Ludovic MellyAnna Marsano
Jul 19, 2006·Tissue Engineering·Seung-Woo ChoByung-Soo Kim
Mar 20, 2010·Circulation Research·Yagna P R Jarajapu, Maria B Grant
Apr 30, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Ombretta PozzoliMaurizio Pesce
Jul 27, 2007·British Journal of Cancer·A Z DudekR P Hebbel
Mar 22, 2006·Pediatric Research·Pasquale GalloGianluigi Condorelli
May 1, 2007·Regenerative Medicine·Pasquale Gallo, Gianluigi Condorelli
Aug 31, 2007·Expert Opinion on Biological Therapy·David T HarrisMohamed A Gaballa
Nov 11, 2015·Scientific Reports·Ivana KosticLino Ferreira
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Marilena LupuJürgen Hescheler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis