Heat Capacity Changes (ΔCp) for Interconversions Between Differentially-Ordered DNA States within Physiological Temperature Domains: Implications for Biological Regulatory Switches

The Journal of Physical Chemistry. B
Jens VölkerKenneth J Breslauer

Abstract

Knowledge of differences in heat capacity changes (ΔCp) between biopolymer states provides essential information about the temperature dependence of the thermodynamic properties of these states, while also revealing insights into the nature of the forces that drive the formation of functional and dysfunctional biopolymer "order." In contrast to proteins, for nucleic acids there is a dearth of direct experimental determination of this information-rich parameter, a deficiency that compromises interpretations of the ever-increasing thermodynamic analyses of nucleic acid properties; particularly as they relate to differential nucleic acid (meta)stability states and their potential biological functions. Here we demonstrate that such heat capacity differences, in fact, exist not only between traditionally measured native to fully unfolded (assumed "random coil") DNA states, but also between competing order-to-order transformations. We illustrate the experimental approach by measuring the heat capacity change between "native"/ordered, sequence homologous, "isomeric" DNA states that differ in conformation but not sequence. Importantly, these heat capacity differences occur within biologically relevant temperature ranges. In short, we d...Continue Reading

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Citations

Aug 21, 2021·The Journal of Physical Chemistry. B·David A NicholsonDavid J Nesbitt

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